Temperature-Dependent Postmortem Changes in Human Cardiac Troponin-T (cTnT): An Approach in Estimation of Time Since Death

Journal of Forensic Sciences
Sachil KumarRaja Rupani

Abstract

Estimation of time of death is an indispensible requirement of every medico-legal autopsy, but unfortunately, there is not a single method by which it could be determined accurately. This study focused on the temperature-dependent postmortem degradation of cardiac troponin-T and its association with postmortem interval (PMI) in human. The analysis involved extraction of the protein, separation by denaturing gel electrophoresis (SDS-PAGE), and visualization by Western blot using cTnT-specific monoclonal antibodies. The area of the bands within a lane was quantified by scanning and digitizing the image using Gel Doc (Universal Hood). The results indicate a characteristic banding pattern among human cadavers (n = 6) and a pseudo-linear relationship between percentage of cTnT degradation and the log of the time since death (r > 0.95), which can be used to estimate the postmortem interval. The data presented demonstrate that this technique can provide an extended time range during which PMI can be more accurately estimated.

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Citations

Mar 10, 2016·International Journal of Legal Medicine·Stefan PittnerPeter Steinbacher
Jan 24, 2017·Journal of Forensic Sciences·Octavio Carvajal-ZarrabalNoé López-Amador
Mar 24, 2018·The American Journal of Forensic Medicine and Pathology·Giuseppe VacchianoMaria Pieri
Mar 14, 2019·International Journal of Legal Medicine·Kyoung-Min ChoiStefan Pittner
Dec 2, 2020·Diagnostics·Angela ZisslerStefan Pittner
Jan 8, 2021·International Journal of Legal Medicine·Jing WangXinshe Liu
Apr 4, 2020·Journal of Proteome Research·Haruka MizukamiNoemi Procopio

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