Temperature-dependent regulation of Thermus thermophilus DnaK/DnaJ chaperones by DafA protein

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Tadashi MizutaniYo-hei Watanabe

Abstract

DafA, a unique 8-kDa protein found in Thermus thermophilus, assembles the chaperones DnaK and DnaJ to produce a DnaK(3)-DnaJ(3)-DafA(3) complex (KJA complex). Although, it is known that DafA is denatured irreversibly at nonphysiological 89 degrees C and the KJA complex dissociates into fully active DnaK and DnaJ, the function of the KJA complex is not fully understood. In this article, we report that the reversible dissociation of the KJA complex occurs in a temperature-dependent manner even below physiological 75 degrees C and that excess DafA completely inhibits the chaperone activities of the DnaK system. The inhibited activities are not rescued by supplementing DnaK or DnaJ. The results indicate that DafA inhibits the chaperone activities of both DnaK and DnaJ by forming the KJA complex and can act as a thermosensor under both heat stress and optimal growth conditions.

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Citations

Feb 22, 2012·The FEBS Journal·Sayaka MizunoYo-hei Watanabe
Oct 8, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yosuke Nakazaki, Yo-Hei Watanabe
Jun 3, 2018·Nature Communications·Takayuki UchihashiToshio Ando

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