Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13.

Marine Drugs
Paris ParedesCarolina Shene

Abstract

Oblongichytrium RT2316-13 synthesizes lipids rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The content of these fatty acids in the total lipids depended on growth temperature. Sequencing technology was used in this work to examine the thraustochytrid's response to a decrease in growth temperature from 15 °C to 5 °C. Around 4% (2944) of the genes were differentially expressed (DE) and only a few of the DE genes (533 upregulated; 206 downregulated) had significant matches to those in the SwissProt database. Most of the annotated DE genes were related to cell membrane composition (fatty acids, sterols, phosphatidylinositol), the membrane enzymes linked to cell energetics, and membrane structure (cytoskeletal proteins and enzymes). In RT2316-13, the synthesis of long-chain polyunsaturated fatty acids occurred through ω3- and ω6-pathways. Enzymes of the alternative pathways (Δ8-desaturase and Δ9-elongase) were also expressed. The upregulation of the genes coding for a Δ5-desaturase and a Δ5-elongase involved in the synthesis of EPA and DHA, explained the enrichment of total lipid with these two long-chain fatty acids at the low temperature. This molecular response has the potential to be used for producing micr...Continue Reading

References

Oct 26, 1976·Biochimica Et Biophysica Acta·R A Demel, B De Kruyff
May 12, 1991·Molecular and Cellular Biochemistry·W H Mager, R J Planta
Jul 8, 1965·Biochimica Et Biophysica Acta·R A Cooper, H L Kornberg
Oct 29, 2003·Chemistry and Physics of Lipids·William Stillwell, Stephen R Wassall
Oct 7, 2004·Journal of Bacteriology·María C MansillaDiego de Mendoza
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Mar 8, 2006·The FEBS Journal·Maria G TozziPiero L Ipata
Jun 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Howard G DamudeNarendra S Yadav
Mar 27, 2007·Journal of Agricultural and Food Chemistry·King-Wai FanFeng Chen
Mar 20, 2009·Journal of Cell Science·Limin Hao, Jonathan M Scholey
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack
Aug 24, 2010·Current Microbiology·M K ChattopadhyayS Shivaji
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Sep 18, 2012·Journal of Biochemistry·Akio Kihara
May 15, 2013·Molecular Systems Biology·Yi-Fan XuJoshua D Rabinowitz
Nov 5, 2013·Biochimica Et Biophysica Acta·Giuseppe ParadiesGiuseppe Petrosillo
Mar 29, 2014·EMBO Reports·Pieter De MaayerDon A Cowan
Jun 11, 2015·Bioinformatics·Felipe A SimãoEvgeny M Zdobnov
Mar 5, 2016·The Plant Journal : for Cell and Molecular Biology·Ryeo Jin KimMi Chung Suh
Sep 16, 2017·Biotechnology Advances·Loris Fossier MarchanTony Gutierrez
Oct 23, 2019·MicrobiologyOpen·Carolina SheneRoberto Armenta

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Citations

Aug 7, 2021·Marine Drugs·Allison LeytonRoberto E Armenta

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Datasets Mentioned

BETA
BAG71007.1
SRR12600987
SRR12600992
PRJNA642733

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Excel
BLASTN
TrimGalore
Geneious
PANTHER
BLAST
GOSeq
RSEM
Trinity
BLAST ( Basic Local Alignment Search Tool )

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