PMID: 9172392Jul 1, 1997Paper

Temperature effects on ion transport across the erythrocyte membrane of the frog Rana temporaria

Comparative Biochemistry and Physiology. Part A, Physiology
N I AgalakovaG P Gusev

Abstract

Unidirectional K+ and Na+ influxes in the frog erythrocytes incubated in Cl- or NO(3)- media with 2.7 mM K+ were measured using 86Rb and 22Na as tracers. K+ influx was inhibited by 35-55% in the presence of 0.2-1.0 mM furosemide but it was unaffected by 0.1-0.2 mM bumetanide. Furosemide at a concentration of 0.5 mM had no effect on K+ loss from the frog red cells incubated in a nominally K(+)-free medium. Together with our previous studies the data support the existence of K-Cl cotransport and the absence of Na-K-2Cl cotransport in the frog erythrocyte membrane. Cell cooling from 20 to 5 degrees C caused a decrease in K+ influx and K+ efflux via the K-Cl cotransporter (3.2- and 3.7-fold, respectively) giving an apparent energy of activation (EA) of about 60 kJ/mol and Q10 value of 2.5. Only small decline (approximately 30%) in the ouabain-sensitive K+ influx was found as temperature was changed from 20 to 5-10 degrees C. Low values of Q10 (approximately 1.5) and EA (27.3 kJ/mol) were obtained for passive K+ influx in the frog erythrocytes (ouabain-insensitive in NO(3)- medium) at temperature within 5-20 degrees C. However, the temperature coefficients were greater for passive Na+ influx and passive K+ efflux (Q10 approximately ...Continue Reading

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Citations

Nov 26, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Gennadii Petrovich Gusev, Natalia Ivanovna Agalakova
May 20, 2016·Intractable & Rare Diseases Research·Juan José González PlazaAinur Akilzhanova
Mar 17, 2011·Human & Experimental Toxicology·S Madusudan RaoT Chandrasekar
Feb 16, 2005·The Journal of Membrane Biology·N C AdragnaP K Lauf
Oct 3, 2018·Advanced Healthcare Materials·Huaifa ZhangJake Barralet

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