Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803

Metabolic Engineering
Tomohisa HasunumaAkihiko Kondo

Abstract

Succinate is a versatile petrochemical compound that can be produced by microorganisms, often from carbohydrate based carbon sources. Phototrophic cyanobacteria including Synechocystis sp. PCC 6803 can more efficiently produce organic acids such as succinate without sugar supplementation, via photosynthetic production of glycogen followed by glycogen utilization, typically under dark conditions. In this study, Synechocystis 6803 bioproduction of organic acids under dark anoxic conditions was found to increase with elevation of temperature from 30 °C to 37 °C. The further enhancement of succinate bioproduction by overexpression of the rate limiting enzyme phosphoenolpyruvate carboxylase resulted in improved glycogen utilization. To gain more insight into the mechanisms underlying the increased organic acid output, a novel temperature dependent metabolomics analysis was performed. Adenylate energy charge was found to decrease along with elevating temperature, while central metabolites glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, glycerol 3-phosphate, malate, fumarate and succinate increased. Temperature dependent 13C-labeling metabolomics analysis further revealed a glycolysis to TCA bottleneck, which cou...Continue Reading

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Citations

Mar 31, 2019·Biotechnology Journal·Peng-Fei XiaMatthew Wook Chang
May 28, 2019·Applied Microbiology and Biotechnology·Sandra Moser, Harald Pichler
Dec 8, 2019·World Journal of Microbiology & Biotechnology·Jiachen ZhaoYingping Zhuang
Apr 17, 2019·Scientific Reports·Shoki ItoTakashi Osanai
Nov 17, 2020·IScience·Damini Jaiswal, Pramod P Wangikar
Sep 2, 2019·Trends in Biotechnology·Christopher J VavrickaAkihiko Kondo
Mar 28, 2021·The Plant Journal : for Cell and Molecular Biology·Leonie Verhage

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