Temperature-induced expression of yeast FKS2 is under the dual control of protein kinase C and calcineurin.

Molecular and Cellular Biology
C ZhaoD E Levin

Abstract

FKS1 and FKS2 are alternative subunits of the glucan synthase complex, which is responsible for synthesizing 1,3-beta-glucan chains, the major structural polymer of the Saccharomyces cerevisiae cell wall. Expression of FKS1 predominates during growth under optimal conditions. In contrast, FKS2 expression is induced by mating pheromone, high extracellular [Ca2+], growth on poor carbon sources, or in an fks1 mutant. Induction of FKS2 expression in response to pheromone, CaCl2, or loss of FKS1 function requires the Ca2+/calmodulin-dependent protein phosphatase calcineurin. Therefore, a double mutant in calcineurin (CNB1) and FKS1 is inviable due to a deficiency in FKS2 expression. To identify novel regulators of FKS2 expression, we isolated genes whose overexpression obviates the calcineurin requirement for viability of an fks1 mutant. Two components of the cell integrity signaling pathway controlled by the RHO1 G protein (MKK1 and RLM1) were identified through this screen. This signaling pathway is activated during growth at moderately high temperatures. We demonstrate that calcineurin and the cell integrity pathway function in parallel, through separable promoter elements, to induce FKS2 expression during growth at 39 degrees C....Continue Reading

References

Jan 1, 1992·Molecular Microbiology·R J Trumbly
Nov 1, 1992·Molecular and Cellular Biology·G ParaviciniM A Payton
Mar 1, 1992·The Journal of Cell Biology·D E Levin, E Bartlett-Heubusch
Oct 10, 1985·Nature·S Munro, H Pelham
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M DouglasM el-Sherbeini
Sep 1, 1995·Microbiological Reviews·V J CidM Sánchez
Aug 1, 1995·European Journal of Biochemistry·S B InoueT Watanabe
Apr 1, 1995·European Journal of Biochemistry·P Shore, A D Sharrocks
Jul 1, 1994·Yeast·F M Klis
Apr 19, 1996·The Journal of Biological Chemistry·Y KamadaD E Levin

❮ Previous
Next ❯

Citations

Oct 6, 1999·The Journal of Cell Biology·P A Delley, M N Hall
Feb 14, 2013·Fungal Genetics and Biology : FG & B·Celia RodríguezJosé M Siverio
Jan 17, 2009·Journal of Bioscience and Bioengineering·Yoshio ArakiHitoshi Shimoi
Apr 3, 2004·Biotechnology Progress·Chi-Kin Chow, Sean P Palecek
Dec 23, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mari HigaReiko Sugiura
Nov 10, 2011·Plant Signaling & Behavior·Thorsten Hamann, Lucy Denness
Mar 13, 2015·BMC Biology·Vincent RobertArturo Casadevall
Jul 3, 2007·Biochimica Et Biophysica Acta·Raymond E Chen, Jeremy Thorner
Aug 12, 1999·Fungal Genetics and Biology : FG & B·S Brul, F M Klis
Oct 18, 2006·FEMS Yeast Research·Isabelle Gagnon-ArsenaultYves Bourbonnais
Dec 4, 2009·PLoS Genetics·Sara E BeeseDavid E Levin
Apr 5, 2008·PLoS Pathogens·Louise A WalkerNeil A R Gow
Nov 19, 2003·Biochemical and Biophysical Research Communications·Peter R Kraus, Joseph Heitman
Nov 2, 2002·Molecular Microbiology·Un Sung JungDavid E Levin
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·B N Lee, E A Elion
Jul 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ran KafriYitzhak Pilpel
Aug 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Raphael H Valdivia, Randy Schekman
Apr 12, 2014·Annals of Botany·Timo Engelsdorf, Thorsten Hamann
Nov 26, 2004·Molecular Biology of the Cell·Quan ZhongMiriam L Greenberg
Jun 2, 2006·Molecular Biology of the Cell·Nan-Nan ZhangKyle W Cunningham

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.