Temperature-induced structural transition in-situ in porcine lens--changes observed in void size distribution

Biochimica Et Biophysica Acta
P SaneJ M Holopainen

Abstract

The function of mammalian ocular lens is to provide a sharp image to the retina. Accordingly, the lens needs to be transparent and minimize light scattering. To do so the lens fiber cells first loose intracellular organelles, organize the cytoplasm and arrange the fiber cell membranes. Because the fiber cells are metabolically inactive, the plasma membrane becomes the only cellular organelle and consequently, the phase behavior of these membranes determines the physiological state of the lens. Previous studies have shown that lipids extracted from the nuclear and cortical region of human lens show a temperature-induced phase transition close to the body temperature. Yet, the physiological function of this phase transition is not known, and even the presence of the phase transition in intact lenses is unknown. Positron annihilation lifetime spectroscopy (PALS) was used to characterize the sub-nanometer-sized local structure of intact porcine lens and these studies were complemented with differential scanning calorimeter and mass spectrometric analysis in extracted porcine lens lipids. Using PALS, we present evidence for the presence of a temperature-dependent structural transition centered at 35.5 degrees C in-situ in clear extr...Continue Reading

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Jul 21, 2009·Biochimica Et Biophysica Acta·Peter J Quinn, Claude Wolf

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Citations

Apr 15, 2010·Journal of Lipid Research·Veronika VidováJuha M Holopainen
May 12, 2011·PloS One·Jaroslav PólJuha M Holopainen
Jul 20, 2010·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·P SaneJ M Holopainen
Jul 28, 2013·Current Eye Research·Alexandra RobciucJuha M Holopainen
May 8, 2015·Physical Chemistry Chemical Physics : PCCP·Celesta FongCalum J Drummond

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