Temperature-jump apparatus with Raman detection based on a solid-state tunable (1.80-2.05 microm) kHz optical parametric oscillator laser

Applied Spectroscopy
Gurusamy BalakrishnanThomas G Spiro

Abstract

The operating characteristics of a pulsed (10 ns) tunable near-infrared (NIR) laser source are described for temperature-jump (T-jump) applications. A Q-switched Nd:YLF laser (approximately 10 ns pulses) with a 1 kHz repetition rate is used to pump a potassium titanyl arsenate (KTA) crystal-based optical parametric oscillator (OPO), producing approximately 1 mJ NIR pulses that are tunable (1.80-2.05 microm) across the 1.9 microm vibrational overtone band of water. This T-jump source has been coupled to a deep ultraviolet (UV) probe laser for Raman studies of protein dynamics. T-jumps of up to 30 degrees C, as measured via the O-H stretching Raman band of water, are readily achieved. Application to cytochrome c unfolding is demonstrated.

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Citations

Oct 4, 2007·Journal of the American Chemical Society·Gurusamy BalakrishnanThomas G Spiro
Nov 15, 2007·Inorganic Chemistry·Thomas G Spiro
Feb 18, 2012·Chemical Reviews·Sulayman A OladepoIgor K Lednev
Sep 1, 2006·Photomedicine and Laser Surgery
Jan 16, 2007·Applied Spectroscopy·Jia Tang, Feng Gai
Apr 2, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jan Kubelka
Mar 8, 2011·The Journal of Physical Chemistry Letters·Sulayman A OladepoSanford A Asher
Oct 26, 2012·Journal of the American Chemical Society·Gurusamy BalakrishnanThomas G Spiro
Oct 6, 2006·The Journal of Physical Chemistry. B·Gurusamy BalakrishnanThomas G Spiro

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