Temperature profiles and heat dissipation in capillary electrophoresis

Analytical Chemistry
Christopher J EvenhuisPaul R Haddad

Abstract

While temperature control is usually employed in capillary electrophoresis (CE) to aid heat dissipation and provide acceptable precision, internal electrolyte temperatures are almost never measured. In principle, this limits the accuracy, repeatability, and method robustness. This work presents a fundamental study that combines the development of new equations characterizing temperature profiles in CE with a new method of temperature determination. New equations were derived from first principles relating the mean, axial, and inner wall electrolyte temperatures (T(Mean), T(Axis), T(Wall)). T(Mean) was shown to occur at a distance 1/ radical3 times the internal radius of the capillary from the center of the capillary and to be a weighted average of (2/3)T(Axis) and (1/3)T(Wall). Conductance (G) and electroosmotic mobility (mu(EOF)) can be used to determine T(Mean) and T(Wall), respectively. Extrapolation of curves of mu(EOF) versus power per unit length (P/L) at different temperatures was used to calibrate the variation of mu(EOF) with temperature (T), free from Joule heating effects. mu(EOF) increased at 2.22%/ degrees C. The experimentally determined temperatures using mu(EOF) agreed to within 0.2 degrees C with those determin...Continue Reading

References

Dec 1, 1967·Chromatographic Reviews·S Hjertén
Oct 21, 1983·Science·J W Jorgenson, K D Lukacs
Sep 19, 2002·Analytical Chemistry·Michael E LaceyJonathan V Sweedler

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Citations

Sep 12, 2007·Electrophoresis·Christopher J EvenhuisPaul R Haddad
Oct 18, 2007·Electrophoresis·Christopher J EvenhuisPaul R Haddad
Dec 7, 2007·Electrophoresis·Xiangchun Xuan
Feb 7, 2009·Electrophoresis·Christopher J Evenhuis, Paul R Haddad
Feb 10, 2009·Electrophoresis·Vlastimil HruskaPaul R Haddad
Sep 29, 2009·Electrophoresis·Violaine PhilippiniChristophe Moulin
Aug 31, 2017·Journal of Chromatography. a·Seung Jae LeeAndreas Manz
Apr 28, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sayyed Hashem SajjadiHossein Ahmadzadeh
May 1, 2013·Analytical Chemistry·Anita Rogacs, Juan G Santiago
May 15, 2015·Analytical Chemistry·Joan M CabotMichael C Breadmore

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