Temperature-Responsive Gene Silencing by a Smart Polymer

Bioconjugate Chemistry
Mingming Wang, Yiyun Cheng

Abstract

Intracellular siRNA release is a crucial step in efficient gene silencing mediated by cationic polymers. Here, we show an example of temperature change-induced intracellular siRNA release and silencing using a temperature-responsive polymer consisting of dendrimer, poly(N-isopropylacrylamide) and phenylboronic acid. The smart polymer can trigger the release of loaded siRNA in a controlled manner upon cooling the surrounding solution below its lower critical solution temperature. Gene silencing efficacy of the polymer was significantly increased by cool treatment after its cellular uptake. The polymer and the cool treatment cause minimal toxicity to the transfected cells. The results provide a facile and promising strategy to design stimuli-responsive polymers for efficient gene silencing.

References

Oct 6, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·M KurisawaT Okano
Feb 26, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Naoya TakedaTeruo Okano
Jul 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·David B RozemaJon A Wolff
Jan 31, 2009·Nature Reviews. Drug Discovery·Kathryn A WhiteheadDaniel G Anderson
Jan 19, 2010·Nature Biotechnology·Sean C SempleMichael J Hope
Feb 8, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ru ChengZhiyuan Zhong
Dec 16, 2011·Nature Materials·Enrico Mastrobattista, Wim E Hennink
Feb 15, 2012·Advanced Drug Delivery Reviews·Min Suk Shim, Young Jik Kwon
Apr 6, 2012·Journal of the American Chemical Society·Stuart S DunnJoseph M DeSimone
Jun 5, 2012·Nature Nanotechnology·Hyukjin LeeDaniel G Anderson
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Erika I GeihePaul A Wender
Feb 23, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Martin PiestJohan F J Engbersen
Oct 24, 2013·Nature Materials·Rosemary KanastyDaniel Anderson
Oct 24, 2013·Nature Materials·Simona MuraPatrick Couvreur
Nov 23, 2013·Journal of Biomaterials Applications·Xiaobo WangJian Shen
Feb 21, 2014·Angewandte Chemie·F PercheV P Torchilin
Jun 16, 2014·Biomaterials·Hong-Xia WangJun Wang
Jun 25, 2013·Advanced Functional Materials·Hongmei LiCraig L Duvall
Dec 3, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Carolin GanasWolfgang J Parak
Jan 9, 2015·Journal of Biomaterials Science. Polymer Edition·Zheng YangAnjie Dong
May 7, 2015·Chemical Reviews·Jiepin YangYiyun Cheng
Jun 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Xi ZhuJinjun Shi
Nov 17, 2015·Journal of the American Chemical Society·Chunyang SunJun Wang

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Citations

Oct 9, 2018·Current Medicinal Chemistry·Ji Hyun RyuShyni Varghese
Jun 26, 2020·Advanced Drug Delivery Reviews·Alexandra S Piotrowski-DaspitW Mark Saltzman

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