PMID: 9545587Apr 18, 1998Paper

Temperature-responsive size-exclusion chromatography using poly(N-isopropylacrylamide) grafted silica

Biochimica Et Biophysica Acta
H LakhiariJ Jozefonvicz

Abstract

Silica-based packing materials induce non-specific interactions with proteins in aqueous media because of the nature of their surface, mainly silanol groups. Therefore, the silica surface has to be modified in order to be used as stationary phase for the High Performance Size-Exclusion Chromatography (HPSEC) of proteins. For this purpose, porous silica beads were coated with hydrophilic polymer gels (dextrans of different molecular weights) carrying a calculated amount of diethyl-aminoethyl groups (DEAE). Actually, as shown by HPSEC, these dextran modified supports minimize non-specific adsorption for proteins and pullulans in aqueous solution. Then, in order to change the pore size in response to temperature, temperature responsive polymer of poly(N-isopropylacrylamide) (PIPAAm) was introduced into the surface of dextran-DEAE on porous silica beads. The structure of these supports before and after modification was alternately studied by Scanning Electronic Microscopy (SEM) and Scanning Force Microscopy (SFM). An adsorption of radiolabelled albumin was performed to complete our study. Silica modifications by dextran-DEAE and PIPAAm improve the neutrality of the support and minimize the non-specific interactions between the soli...Continue Reading

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Citations

Apr 17, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·H LakhiariD Muller
Nov 7, 2009·Journal of Pharmaceutical Sciences·Tsutomu ArakawaJohn S Philo
Sep 3, 2004·Colloids and Surfaces. B, Biointerfaces·Malin Jönsson, Hans-Olof Johansson
Sep 4, 2013·Advanced Materials·Ekaterina V Skorb, Helmuth Möhwald
Jul 9, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hamid Lakhiari, Daniel Muller
Jul 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weiwei SunAiqin Luo
Sep 22, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Sina BurkertManfred Stamm
Mar 5, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Havazelet Bianco-Peled, Shlomit Gryc
Jul 19, 2003·Science·Dale L HuberBruce C Bunker

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