Temperature-sensitive activation of G-protein regulating the resting membrane conductance of Aplysia neurons

The Japanese Journal of Physiology
S HakozakiK Sasaki

Abstract

Raising the temperature from 22 to 32 degrees C induced a marked hyperpolarization (15-30 mV) associated with an increase in membrane conductance of Aplysia neurons, whereas lowering the temperature from 22 to 12 degrees C caused a significant depolarization (10-20 mV) with a decrease in conductance. These temperature effects were far greater than those expected from the Nernst equation. The reversal potentials of these temperature responses corresponded with the equilibrium potential of K+, suggesting these responses were produced by opening or closing of K+ channels. Ouabain (5 x 10(-4) M) did not affect these temperature responses though it depolarized all cells examined (5-25 mV). Intracellularly injected guanosine 5'-O-(2-thiodiphosphate) (GDP beta S) selectively depressed the response to warming without affecting the response to cooling. Intracellular application of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) produced a gradual increase in K+ conductance of the resting membrane and apparently depressed the response to warming while it markedly augmented the response to cooling. These results suggest that GTP binding protein can be activated thermally to open K+ channels without receptor stimulation. It is significan...Continue Reading

Citations

Nov 1, 1993·The Journal of Membrane Biology·Y H Chen, R L DeHaan
Mar 1, 2015·Pflügers Archiv : European journal of physiology·Tom A CoatesMark D Baker
Jan 31, 2012·Neuro-Signals·Nam Gyu HyunBong-Kiun Kaang
Feb 24, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Nam Gyu HyunBong-Kiun Kaang

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