Temperature-sensitive, colicin M-tolerant mutant of Escherichia coli.

Journal of Bacteriology
K SchallerV Braun

Abstract

A mutant sensitive to colicin M at 30 degrees C and tolerant at 42 degrees C to high concentrations of colicin M was isolated from Escherichia coli K-12. A temperature shift from 30 to 42 degrees C rescued all cells up to the time they started to lyse at 30 degrees C (25 min after addition of colicin M). The growth rate at 42 degrees C remained unaffected by colicin M. AT 42 degrees C the cell-bound colicin M was inactivated by trypsin, sodium dodecyl sulfate, and antiserum against colicin M. Ferrichrome competed with colicin M at 42 degrees C only during the initial adsorption to the common receptor protein in the outer membrane. Since cells lysed earlier at 30 degrees C when they had been preincubated with colicin M at 42 degrees C, we conclude that the process leading finally to cell lysis is initiated at 42 degrees C and stops at a later stage of colicin M trypsin, dodecyl sulfate, and antiserum when cells were transferred from 30 to 42 degrees C, we assume that colicin M is translocated from its target site towards the cell surface. The mutation conferring tolerance was mapped close to the rpsL gene.

References

Jul 1, 1978·Journal of Bacteriology·K Hantke, V Braun
Jan 1, 1975·FEBS Letters·K Hantke, V Braun
Dec 1, 1975·Bacteriological Reviews·K G Hardy
Oct 1, 1967·Journal of Bacteriology·R Nagel de Zwaig, S E Luria
May 1, 1974·Antimicrobial Agents and Chemotherapy·V BraunM R Wabl
Jan 1, 1967·Annual Review of Microbiology·M Nomura
Jul 1, 1980·Journal of Bacteriology·R J KadnerV Braun
Apr 1, 1980·Journal of Bacteriology·V BraunK Schaller
Apr 1, 1981·Journal of Bacteriology·K SchallerV Braun

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Citations

Jan 1, 1984·Molecular & General Genetics : MGG·T OlschlägerV Braun
Mar 9, 1999·Folia Microbiologica·J Smarda, D Smajs
Nov 28, 2012·Biochemical Society Transactions·Volkmar BraunSilke I Patzer
Jun 17, 2008·Molecular Microbiology·Julia HullmannVolkmar Braun
Jan 1, 1985·Archives of Microbiology·R DreherB Wittmann-Liebold
Jul 1, 1991·FEMS Microbiology Reviews·R E Harkness, T Olschläger
Mar 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Hélène BarreteauDominique Mengin-Lecreulx
Jul 22, 2008·The Journal of Biological Chemistry·Kornelius ZethVolkmar Braun
Sep 12, 2018·Annual Review of Microbiology·Volkmar Braun
Nov 9, 2002·Biochimie·Volkmar BraunKlaus Hantke
Dec 1, 1982·Journal of Bacteriology·K SchallerV Braun
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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