Temperature-sensitive repression of the tryptophan operon in Escherichia coli.

Journal of Bacteriology
K ItoT Yura

Abstract

Mutants of Escherichia coli exhibiting temperature-sensitive repression of the tryptophan operon have been isolated among the revertants of a tryptophan auxotroph, trpS5, that produces an altered tryptophanyl transfer ribonucleic acid (tRNA) synthetase. Unlike the parental strain, these mutants grew in the absence of tryptophan at high but not at low temperature. When grown at 43.5 C with excess tryptophan (repression conditions), they produced 10 times more anthranilate synthetase than when grown at 36 C or lower temperatures. Similar, though less striking, temperature-sensitivity was observed with respect to the formation of tryptophan synthetase. Transduction mapping by phage P1 revealed that these mutants carry a mutation cotransducible with thr at 60 to 80%, in addition to trpS5, and that the former mutation is primarily responsible for the temperature-sensitive repression. These results suggest that the present mutants represent a novel type of mutation of the classical regulatory gene trpR, which probably determines the structure of a protein involved in repression of the tryptophan operon. In agreement with this conclusion, tRNA of several trpR mutants was found to be normal with respect to its tryptophan acceptability....Continue Reading

References

Mar 9, 1967·Biochemical and Biophysical Research Communications·S HiragaT Yura
Apr 1, 1968·Journal of Bacteriology·W F Doolittle, C Yanofsky
Dec 1, 1968·Journal of Molecular Biology·D E Morse, C Yanofsky
Jan 14, 1969·Journal of Molecular Biology·S Hiraga
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·N SueokaW J Gartland
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·R H Bauerle, P Margolin
Aug 31, 1962·Biochemical and Biophysical Research Communications·J A GALLANT
Apr 9, 1965·Biochemical and Biophysical Research Communications·S UDAKA, T HORIUCHI
Jun 1, 1965·Journal of Molecular Biology·J R SADLER, A NOVICK
Oct 1, 1961·Journal of Molecular Biology·T HORIUCHIA NOVICK

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Citations

Mar 1, 1976·Bacteriological Reviews·B J BachmannA L Taylor
Jun 1, 1970·Bacteriological Reviews·A L Taylor
Sep 15, 1973·Journal of Molecular Biology·S Hiraga, C Yanofsky
Mar 1, 1974·Science·R F Goldberger

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