PMID: 1203258Dec 19, 1975Paper

Temperature sensitivity of protein synthesis initiation in the reticulocyte lysate system. Reduced formation of the 40 S ribosomal subunit - Met-tRNAf complex at an elevated temperature

Biochimica Et Biophysica Acta
S Mizuno

Abstract

Analysis of protein synthesis in the rabbit reticulocyte lysate system revealed the existence of a temperature-sensitive step in chain initiation which became irreversibly inactivated in the incubation at 42 degrees C. This inactivation of initiation was accompanied by a marked reduction in formation of the 40 S ribosomal subunit - Met-tRNAf complex. Decreased protein synthesis and a decrease in formation of the 40 S complex were also evident in unfortified lysates which had been prewarmed at 42 degrees C prior to protein synthesis. Hemin did not protect such lysates. The addition of supernatant fraction of a fresh lysate did not promote recovery of the reduced protein synthesis by such prewarmed lysates. Moreover, the addition of supernatant fraction prewarmed at 42 degrees C in the presence of added hemin caused little inhibition of protein synthesis by fresh lysate. The results indicate that the supernatant fraction is not involved in the inactivation.

References

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Citations

Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A E HaschemeyerM A Smith
Oct 1, 1984·The Journal of Cell Biology·R Morimoto, E Fodor
Mar 15, 1978·European Journal of Biochemistry·S A Bonanou-TzedakiH R Arnstein
Nov 1, 1984·Molecular and Cellular Biology·S S BanerjiR I Morimoto

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