Temperature threshold and preservation of signaling for mitochondrial membrane proteins during ischemia in rabbit heart

Cryobiology
X H NingM A Portman

Abstract

Temperature modulates both myocardial energy requirements and production. We have previously demonstrated that myocardial protection induced by hypothermic adaptation preserves expression of genes regulating heat shock protein and the nuclear-encoded mitochondrial proteins, the adenine nucleotide translocator isoform 1 (ANT1), and the beta subunit of F1-ATPase (beta F1-ATPase). This preservation is associated with a reduction in ATP depletion similar to that noted in cardioplegic arrested hearts preserved at a critical temperature (30 degrees C) or below. We tested the hypothesis that expression of these genes may also be subject to this temperature threshold phenomenon. Isolated perfused rabbit hearts were subjected to ischemic cardioplegic arrest at 4, 30, or 34 degrees C for 120 min. Cardiac function indices and steady-state mRNA levels for ANT1, beta F1-ATPase, and HSP70-1 were measured prior to ischemia (B) and after 45 min of reperfusion. Cardiac function was significantly depressed in the 34 degrees C group. Ischemia at 34 degrees C reduced steady-state mRNA levels for ANT1 and beta F1-ATPase from B, but these levels were similarly preserved at 4 and 30 degrees C. HSP70-1 levels were mildly elevated (fourfold) above B to...Continue Reading

References

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Citations

Apr 7, 2010·American Journal of Physiology. Heart and Circulatory Physiology·David G BeiserTerry L Vanden Hoek
Sep 26, 2000·Molecular Genetics and Metabolism·M A Portman
Dec 17, 2014·Therapeutic Hypothermia and Temperature Management·Xue-Han NingMichael A Portman
Sep 5, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Xue-Han NingMichael A Portman
Mar 15, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Xue-Han NingMichael A Portman
Jul 31, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Xue-Han NingMichael A Portman
Mar 5, 2021·Frontiers in Cardiovascular Medicine·Kelly P YamadaKiyotake Ishikawa

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