Template-free 2D particle fusion in localization microscopy

Nature Methods
Hamidreza HeydarianBernd Rieger

Abstract

Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.

References

Jul 25, 2006·Journal of Structural Biology·Guang TangSteven J Ludtke
Feb 24, 2010·Journal of Biotechnology·Sebastian van de LindeMarkus Sauer
Apr 7, 2010·Nature Methods·Carlas S SmithKeith A Lidke
Dec 22, 2010·IEEE Transactions on Pattern Analysis and Machine Intelligence·Bing Jian, Baba C Vemuri
May 12, 2011·Biomedical Optics Express·Fang HuangKeith A Lidke
Apr 30, 2013·Nature Methods·Robert P J NieuwenhuizenBernd Rieger
Oct 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Richard Henderson
Jan 18, 2014·Science·Schuyler B Van EngelenburgJennifer Lippincott-Schwartz
Feb 6, 2014·Histochemistry and Cell Biology·Teresa KleinMarkus Sauer
Jan 1, 2012·Computational and Structural Biotechnology Journal·Mikhail KudryashevHenning Stahlberg
Apr 14, 2015·Methods and Applications in Fluorescence·Jordi BroekenBernd Rieger
May 21, 2015·PloS One·Robert P J NieuwenhuizenSjoerd Stallinga
Jul 4, 2015·Histochemistry and Cell Biology·Anne BurgertMarkus Sauer
Jan 13, 2017·Nature Communications·Patrick Fox-RobertsSusan Cox
May 19, 2017·Nature Protocols·Joerg SchnitzbauerRalf Jungmann
Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Desirée SalasMarcelo Nollmann
Apr 25, 2018·Nature Communications·Maximilian T StraussRalf Jungmann

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Citations

Dec 11, 2019·Nature Methods·Jelmer CnossenSjoerd Stallinga
Sep 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ji Yu, Ahmed Elmokadem
Mar 25, 2020·Biomedical Optics Express·Peter KnerSjoerd Stallinga
Oct 3, 2018·Nature Methods·Mark Bates
Dec 1, 2020·Nano Letters·Alistair P CurdMichelle Peckham
Mar 22, 2021·Ageing Research Reviews·Nancy AdamKarl Riabowol
Aug 25, 2020·Trends in Cell Biology·Yu-Le WuJonas Ries
May 13, 2019·Methods : a Companion to Methods in Enzymology·Angélique JimenezChristophe Leterrier
May 16, 2021·Nature Communications·Hamidreza HeydarianBernd Rieger
Jun 20, 2021·Nature Communications·Teun A P M HuijbenBernd Rieger
Jul 7, 2021·ACS Nano·Rishabh M ShettyAshwin Gopinath

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Methods Mentioned

BETA
electrophoresis
PCA

Software Mentioned

mathop
EMAN
STORM

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