Temporal analysis of Arabidopsis genes activated by Eucalyptus grandis NAC transcription factors associated with xylem fibre and vessel development

Scientific Reports
M LaubscherSteven G Hussey

Abstract

Secondary cell wall (SCW) deposition in Arabidopsis is regulated among others by NAC transcription factors, where SND1 chiefly initiates xylem fibre differentiation while VND6 controls metaxylem vessel SCW development, especially programmed cell death and wall patterning. The translational relevance of Arabidopsis SCW regulation theory and the utility of characterized transcription factors as modular synthetic biology tools for improving commercial fibre crops is unclear. We investigated inter-lineage gene activation dynamics for potential fibre and vessel differentiation regulators from the widely grown hardwood Eucalyptus grandis (Myrtales). EgrNAC26, a VND6 homolog, and EgrNAC61, an SND1 homolog, were transiently expressed in Arabidopsis mesophyll protoplasts in parallel to determine early and late (i.e. 7 and 14 hours post-transfection) gene targets. Surprisingly, across the time series EgrNAC26 activated only a subset of SCW-related transcription factors and biosynthetic genes activated by EgrNAC61, specializing instead in targeting vessel-specific wall pit and programmed cell death markers. Promoters of EgrNAC26 and EgrNAC61 both induced reporter gene expression in vessels of young Arabidopsis plants, with EgrNAC61 also c...Continue Reading

References

Mar 1, 1996·Current Biology : CB·W ChiuJ Sheen
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Mar 1, 1964·The Journal of Cell Biology·P K HEPLER, E H NEWCOMB
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Aug 24, 2004·Plant Cell Reports·Antanas V SpokeviciusGerd Bossinger
Aug 16, 2005·The Plant Journal : for Cell and Molecular Biology·Monica GoicoecheaJacqueline Grima-Pettenati
Aug 17, 2005·Genes & Development·Minoru KuboTaku Demura
Jan 17, 2007·Trends in Plant Science·Taku Demura, Hiroo Fukuda
Oct 4, 2007·Journal of Cell Science·Pascale RossignolJohn H Doonan
May 1, 2008·The Plant Journal : for Cell and Molecular Biology·Masatoshi YamaguchiTaku Demura
Jun 25, 2008·The Plant Journal : for Cell and Molecular Biology·Utku AvciCandace H Haigler
Mar 28, 2009·International Journal of Molecular Sciences·Donald L RockwoodJerrold E Winandy
Aug 14, 2009·The Plant Journal : for Cell and Molecular Biology·Jae-Heung KoKyung-Hwan Han
Apr 13, 2010·Current Opinion in Plant Biology·Taku Demura, Zheng-Hua Ye
Apr 13, 2010·Plant Signaling & Behavior·Ruiqin Zhong, Zheng-Hua Ye
Jun 22, 2010·Bioinformatics·Daniel BlankenbergUNKNOWN Galaxy Team
Jul 2, 2010·Annual Review of Cell and Developmental Biology·Ana Caño-DelgadoTaku Demura
Sep 28, 2010·Current Opinion in Plant Biology·Kyoko Ohashi-Ito, Hiroo Fukuda
Dec 15, 2010·Bioinformatics·Michael E SmootTrey Ideker
Feb 3, 2011·The Plant Journal : for Cell and Molecular Biology·Masatoshi YamaguchiTaku Demura
Jun 9, 2011·The Plant Journal : for Cell and Molecular Biology·Misato OhtaniTaku Demura
Sep 3, 2011·The Plant Journal : for Cell and Molecular Biology·Huanzhong WangRichard A Dixon

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP117192

Methods Mentioned

BETA
GTPase
RNA-seq
confocal microscopy
transgenic
electrophoretic mobility shift
transfection
transfections
transgenics

Software Mentioned

FastQC
R
Cufflinks
FastQGroomer
Cytoscape

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis