Temporal and molecular dynamics of human metastatic breast carcinoma cell adhesive interactions with human bone marrow endothelium analyzed by single-cell force spectroscopy

PloS One
Leike XieV V Glinsky

Abstract

Bone is a common site of metastasis for breast cancer and the mechanisms of metastasis are not fully elucidated. The purpose of our study was to characterize temporal and molecular dynamics of adhesive interactions between human breast cancer cells (HBCC) and human bone marrow endothelium (HBME) with piconewton resolution using atomic force microscopy (AFM). In adhesion experiments, a single breast cancer cell, MDA-MB-231 (MB231) or MDA-MB-435 (MB435) was attached to the AFM cantilever and brought into contact with a confluent HBME monolayer for different time periods (0.5 to 300 sec). The forces required to rupture individual molecular interactions and completely separate interacting cells were analyzed as measures of cell-cell adhesion. Adhesive interactions between HBME and either MB231 or MB435 cells increased progressively as cell-cell contact time was prolonged from 0.5 to 300 sec due to the time-dependent increase in the number and frequency of individual adhesive events, as well as to the involvement of stronger ligand-receptor interactions over time. Studies of the individual molecule involvement revealed that Thomsen-Friedenreich antigen (TF-Ag), galectin-3, integrin-β1, and integrin-α3 are all contributing to HBCC/HB...Continue Reading

References

Jul 25, 2000·European Journal of Clinical Investigation·P M RoodC E van der Schoot
Mar 17, 2004·The Journal of Cell Biology·Hui WangRuth J Muschel
Jan 1, 2005·The Journal of Membrane Biology·Tsutomu Tsuji
Apr 22, 2005·Apoptosis : an International Journal on Programmed Cell Death·S NakaharaA Raz
Jun 22, 2005·Neoplasia : an International Journal for Oncology Research·Olga V GlinskiiVladislav V Glinsky
Aug 16, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Zhe SunGerald A Meininger
Oct 6, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Senthil R KumarSusan L Deutscher
May 6, 2006·Ultramicroscopy·Pierre-Henri PuechDaniel J Muller
Aug 8, 2006·Nature Medicine·Patricia S Steeg
Sep 2, 2006·Experimental Biology and Medicine·Xiaohui ZhangVincent T Moy
Oct 26, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert E Coleman
Nov 30, 2006·Neoplasia : an International Journal for Oncology Research·Jamie HeimburgKate Rittenhouse-Olson
Dec 16, 2006·Cancer Metastasis Reviews·Vladislav V Glinsky
Jul 29, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Rita SilvaLouise E Reynolds
May 6, 2009·Annual Review of Biomedical Engineering·Konstantinos Konstantopoulos, Susan N Thomas
Sep 24, 2011·The Journal of Biological Chemistry·Claudia Tanja Mierke
Oct 18, 2011·Cell·Scott Valastyan, Robert A Weinberg
Dec 29, 2011·Frontiers in Bioscience (Scholar Edition)·Adel AlmogrenKate Rittenhouse-Olson
Apr 10, 2012·Seminars in Cancer Biology·Daniela SpanoMassimo Zollo
Apr 17, 2012·International Journal of Cell Biology·Gerd Bendas, Lubor Borsig
Feb 12, 2013·Methods : a Companion to Methods in Enzymology·Jens FriedrichsMartin Benoit
Apr 26, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shankar J EvaniAnand K Ramasubramanian
Oct 19, 2013·Oncology Letters·Toni IbrahimDino Amadori
Mar 11, 2015·Breast Cancer Research : BCR·Attaya Suvannasankha, John M Chirgwin

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Citations

Apr 1, 2020·Journal of Molecular Recognition : JMR·Leonardo VenturelliSandor Kasas

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Datasets Mentioned

BETA
MDA-MB-435

Methods Mentioned

BETA
AFM

Software Mentioned

IGOR Pro
MATLAB
Origin

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