Temporal and spatial distribution of macrophage phenotype markers in the foreign body response to glutaraldehyde-crosslinked gelatin hydrogels

Journal of Biomaterials Science. Polymer Edition
Tony YuKara L Spiller

Abstract

Currently, it is not well understood how changes in biomaterial properties affect the foreign body response (FBR) or macrophage behavior. Because failed attempts at biomaterial degradation by macrophages have been linked to frustrated phagocytosis, a defining feature of the FBR, we hypothesized that increased hydrogel crosslinking density (and decreased degradability) would exacerbate the FBR. Gelatin hydrogels were crosslinked with glutaraldehyde (0.05, 0.1, and 0.3%) and implanted subcutaneously in C57BL/6 mice over the course of 3 weeks. Interestingly, changes in hydrogel crosslinking did not affect the thickness of the fibrous capsule surrounding the hydrogels, expression of the pan-macrophage marker F480, expression of three macrophage phenotype markers (iNOS, Arg1, CD163), or expression of the myofibroblast marker aSMA, determined using semi-quantitative immunohistochemical analysis. With respect to temporal changes, the level of expression of the M1 marker (iNOS) remained relatively constant throughout the study, while the M2 markers Arg1 and CD163 increased over time. Expression of these M2 markers was highly correlated with fibrous capsule thickness. Differences in spatial distribution of staining also were noted, with...Continue Reading

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Citations

Mar 10, 2017·Seminars in Immunology·Aaron H MorrisT R Kyriakides
Jan 19, 2019·Advanced Healthcare Materials·Claire E WitherelKara L Spiller
Feb 5, 2019·Advanced Healthcare Materials·Andrew T RowleyWendy F Liu
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Jun 27, 2020·Scientific Reports·Josephine Y FangBo Han
Jan 29, 2020·Journal of Materials Science. Materials in Medicine·Fabian Obregon-MianoAxel Spahr
Dec 18, 2020·Frontiers in Chemistry·Talaial B AlinaKara L Spiller
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Yining Liu, Tatiana Segura
Jan 16, 2021·Biomaterials·Claire E WitherelKara L Spiller
Feb 25, 2021·ACS Biomaterials Science & Engineering·Sutton E WheelisDanieli C Rodrigues
May 20, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Daniel HachimBryan N Brown
Jun 4, 2021·Acta Biomaterialia·Jingyi HouHai-Quan Mao
Apr 27, 2018·Biomacromolecules·Lauren E JansenShelly R Peyton
Aug 21, 2021·Chemical Reviews·Ricardo WhitakerKara L Spiller
Apr 9, 2018·ACS Biomaterials Science & Engineering·Pamela L GraneyKara L Spiller
Apr 9, 2018·ACS Biomaterials Science & Engineering·Claire E WitherelKara L Spiller
May 13, 2019·ACS Biomaterials Science & Engineering·Javad JafariAndrea J O'Connor
May 21, 2017·Advanced Drug Delivery Reviews·Kara L Spiller, Timothy J Koh
Oct 3, 2020·Ocular Oncology and Pathology·Kristina Joana SchoellesClaudia Auw-Haedrich

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