Temporal and spatial dynamics of the periodic increase in intracellular free calcium at fertilization of golden hamster eggs

Developmental Biology
S MiyazakiY Hiramoto

Abstract

A series of periodic increases in intracellular free calcium concentration ([Ca2+]i) occurred upon fertilization in golden hamster eggs. The spatial distribution of the Ca2+ transients was investigated in single zona-free, aequorin-injected eggs, inseminated by single sperm. A supersensitive TV camera system for recording Ca2+-aequorin luminescence enabled us to observe the spatial distribution of the Ca2+ rise. In the first response, which usually occurred 10-30 sec after the sperm attachment, the increase in [Ca2+]i began near the sperm attachment site, and the Ca2+ rise spread over the entire egg within 4-7 sec. The Ca2+ rise attained its peak in 5-8 sec, declined with almost even distribution, and ceased in 12-17 sec. The spreading Ca2+ rise was repeated in the second and sometimes the third response, starting from the same focus, but spreading more rapidly (approximately 2 sec). In succeeding responses [Ca2+]i increased synchronously in the whole cytoplasm within 1 sec. When additional sperm attached to the egg after the occurrence of the first response by the first sperm, the spread of the Ca2+ rise could take place from near the site of additional sperm attachment but only in the second or third response.

References

Feb 1, 1977·Circulation Research·A Fabiato, F Fabiato
Dec 1, 1978·Biology of Reproduction·R Yanagimachi
Jan 1, 1987·Developmental Biology·H Y KubotaY Hiramoto
Apr 1, 1985·The Journal of Cell Biology·W B Busa, R Nuccitelli
May 1, 1985·The Journal of Cell Biology·A Eisen, G T Reynolds
Mar 1, 1969·Journal of Reproduction and Fertility·R Yanagimachi
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S Miyazaki, Y Igusa
Nov 1, 1984·The Journal of Cell Biology·A Eisen, G T Reynolds
Dec 24, 1981·Nature·K S CuthbertsonP H Cobbold
Feb 1, 1978·The Journal of Cell Biology·J C GilkeyG T Reynolds

❮ Previous
Next ❯

Citations

Dec 1, 1996·Molecular Reproduction and Development·P DamianiR T Duby
Feb 1, 1997·Molecular Reproduction and Development·H WuR A Fissore
Apr 1, 1997·Molecular Reproduction and Development·G I GallicanoD G Capco
Jul 1, 1997·Molecular Reproduction and Development·A J DiMaggioJ Stewart-Savage
Oct 10, 1997·Molecular Reproduction and Development·H IgarashiK Doi
Dec 24, 1997·Molecular Reproduction and Development·H WuR A Fissore
May 7, 1999·Molecular Reproduction and Development·W H WangB N Day
Feb 5, 2000·Molecular Reproduction and Development·T TakahashiE Takahashi
Aug 26, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Tesarik, C Mendoza
May 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K Swann, F A Lai
Mar 1, 1991·Journal of Electron Microscopy Technique·F J Longo
Dec 1, 1991·Molecular Reproduction and Development·J Stewart-Savage, B D Bavister
Jan 1, 1993·Pflügers Archiv : European journal of physiology·K FuruyaS Yamagishi
Feb 17, 1993·Molecular and Cellular Biochemistry·M Konishi, S Kurihara
Mar 25, 2011·Pflügers Archiv : European journal of physiology·Bo Yeun KimDong Ryul Lee
Jan 1, 1987·Developmental Biology·H Y KubotaY Hiramoto
Aug 1, 1987·Developmental Biology·R Nuccitelli
Sep 1, 1989·Developmental Biology·J E SpeksnijderL F Jaffe
Nov 1, 1990·Developmental Biology·J E SpeksnijderL F Jaffe
Jun 1, 1987·Cell Differentiation·C Sardet, P Chang
May 1, 1993·Theriogenology·R ProcházkaG J Marcus
Jan 1, 1991·Pharmacology & Therapeutics·T A Rooney, A P Thomas
May 22, 1990·Biochimica Et Biophysica Acta·R Jacob
Feb 1, 1995·Cellular Signalling·N TakuwaY Takuwa
Nov 1, 1989·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·M Charbonneau, N Grandin
Jul 23, 1999·Life Sciences·Y KurodaK Mikoshiba
Oct 16, 2002·Cell Calcium·L Jaffe
Sep 16, 2000·Molecular and Cellular Endocrinology·S HeyersM Montag
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T Meyer, L Stryer
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L F Jaffe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.