Temporal and spatial expression of fibroblast growth factor receptor 4 isoforms in murine tissues

The Histochemical Journal
Simon CoolVictor Nurcombe

Abstract

Fibroblast growth factor receptors (FGFRs) undergo highly regulated spatial and temporal changes of expression during development. This study describes the use of quantitative reverse transcriptase-polymerase chain reaction and immunochemistry to assess the changes in expression of FGFR4 as compared to its FGFR4-17a and -17b isoforms in mouse tissues, from early embryogenesis through to adulthood. Compared to FGFR4, the expression of the isoforms is more restricted at all developmental stages tested. The reverse transcriptase-polymerase chain reaction demonstrated that FGFR4 is expressed in more tissue types than either of its isoforms: it was found predominantly in lung, liver, brain, skeletal muscle and kidney, whereas the FGFR4-17a form was detected in lung and skeletal muscle, and the FGFR4-17b form only in lung, liver, skeletal muscle and kidney. Immunohistochemistry confirmed strong FGFR4-17b expression in the postnatal lung. When combined, the results suggest that FGFR4 variants play important roles particularly in lung and skeletal muscle development.

Citations

Apr 15, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Alexandra A BlakWolfgang Wurst
Mar 20, 2010·Circulation Research·Harold E Olivey, Eric C Svensson
Jan 23, 2010·American Journal of Respiratory and Critical Care Medicine·Sorachai SrisumaThomas J Mariani
Sep 4, 2020·Signal Transduction and Targeted Therapy·Yangli XieLin Chen

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