Temporal and spatial localization of nectin-1 and l-afadin during synaptogenesis in hippocampal neurons

The Journal of Comparative Neurology
Seung T LimHoward J Federoff

Abstract

Nectins are cell adhesion molecules that, together with the intracellular binding partner afadin, mediate adhesion and signaling at a variety of intercellular junctions. In this work we studied the distribution of nectin-1 and afadin during hippocampal synapse formation using cultured primary hippocampal neurons. Nectin-1 and afadin cluster at developing synapses between hippocampal neurons. These nectin-afadin clusters uniformly colocalize with N-cadherin-catenin pairs, suggesting that formation of developing synapses involves participation of both bimolecular systems. Nectin-1 is initially expressed at excitatory and inhibitory synapses but is progressively lost at inhibitory synapses during their maturation. Treatment of neurons with actin depolymerizing agents disrupts the synaptically localized nectin-1 and afadin cluster at an early stage and elicits nectin-1 ectodomain shedding. These data indicate that the synaptic localization of nectin-1 and l-afadin are F-actin-dependent and that the shedding of nectin-1 is a mechanism contributing to synaptic plasticity.

References

Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S Trimmer
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J G Izant, J R McIntosh
Jan 1, 1994·Annual Review of Neuroscience·A M Craig, G Banker
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·S J SiegelJ H Morrison
Jan 12, 1996·The Journal of Biological Chemistry·M KuriyamaK Kaibuchi
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·B HockK Strebhardt
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J Y SongN Brose
May 21, 1999·Biochemical and Biophysical Research Communications·T YamamotoK Kaibuchi
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·B BoettnerL Van Aelst
Nov 15, 2000·The Journal of Biological Chemistry·U SteinhusenO Huber
Feb 6, 2002·The Journal of Cell Biology·Akira MizoguchiYoshimi Takai
Mar 22, 2002·The Journal of Biological Chemistry·Sabine PokuttaWilliam I Weis
Oct 2, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Atsunori FukuharaYoshimi Takai
Nov 20, 2002·The Journal of Cell Biology·Matthew N RasbandJames S Trimmer
Nov 26, 2002·Biochemical and Biophysical Research Communications·Yoshinari TanakaYoshimi Takai
Feb 1, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomoyuki HondaYoshimi Takai
Jun 6, 2003·Biochemical and Biophysical Research Communications·Tomoyuki HondaYoshimi Takai
Jun 17, 2003·Molecular and Cellular Biology·G RadziwillK Moelling

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Citations

May 27, 2010·The Journal of Biological Chemistry·Jinsook KimSeung T Lim
Feb 10, 2009·Human Gene Therapy·Yahui Grace ChiuHoward J Federoff
May 18, 2012·Molecular Biology of the Cell·Nariaki IwasawaIzumi Oinuma
Feb 26, 2016·Frontiers in Cell and Developmental Biology·Iryna Leshchyns'ka, Vladimir Sytnyk
Apr 24, 2014·Protein & Cell·Xiaofei YangChen Zhang
Apr 15, 2009·Developmental Neurobiology·Nazlie S LatefiEdward S Ruthazer
Sep 14, 2011·Journal of Neurochemistry·Jinsook KimSeung T Lim
Nov 29, 2011·Journal of Neurochemistry·Seung T LimHoward J Federoff
Mar 27, 2015·Molecular Biology of the Cell·Kentaro UmedaIzumi Oinuma
Mar 20, 2012·PloS One·Elise C LelièvreHélène Birbes
May 7, 2013·Nature Neuroscience·Xiao-Dong WangMathias V Schmidt
Apr 18, 2009·Cell Adhesion & Migration·Hideru TogashiYoshimi Takai
Jul 28, 2019·Neuromolecular Medicine·Yan Jun Lee, Toh Hean Ch'ng
Nov 9, 2020·Neural Development·Johanna TomorskyCristopher M Niell
Dec 16, 2020·The Journal of Comparative Neurology·Rio IkutaMami Kurumata-Shigeto
Jan 14, 2021·Cells·David de Agustín-DuránCristina Gil-Sanz
Oct 12, 2021·Molecular and Cellular Biochemistry·Kiyohito MizutaniYoshimi Takai

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