Temporal and Spatial Resolution of Activated Plant Defense Responses in Leaves of Nicotiana benthamiana Infected with Dickeya dadantii

Frontiers in Plant Science
María Luisa Pérez-BuenoMatilde Barón

Abstract

The necrotrophic bacteria Dickeya dadantii is the causal agent of soft-rot disease in a broad range of hosts. The model plant Nicotiana benthamiana, commonly used as experimental host for a very broad range of plant pathogens, is susceptible to infection by D. dadantii. The inoculation with D. dadantii at high dose seems to overcome the plant defense capacity, inducing maceration and death of the tissue, although restricted to the infiltrated area. By contrast, the output of the defense response to low dose inoculation is inhibition of maceration and limitation in the growth, or even eradication, of bacteria. Responses of tissue invaded by bacteria (neighboring the infiltrated areas after 2-3 days post-inoculation) included: (i) inhibition of photosynthesis in terms of photosystem II efficiency; (ii) activation of energy dissipation as non-photochemical quenching in photosystem II, which is related to the activation of plant defense mechanisms; and (iii) accumulation of secondary metabolites in cell walls of the epidermis (lignins) and the apoplast of the mesophyll (phytoalexins). Infiltrated tissues showed an increase in the content of the main hormones regulating stress responses, including abscisic acid, jasmonic acid, and s...Continue Reading

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Citations

Sep 15, 2016·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Matilde BarónMaría Luisa Pérez-Bueno
Mar 2, 2018·Frontiers in Plant Science·Mónica PinedaMatilde Barón
Oct 18, 2019·Frontiers in Plant Science·María Luisa Pérez-BuenoMatilde Barón
Dec 21, 2016·Frontiers in Plant Science·María L Pérez-BuenoMatilde Barón

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Methods Mentioned

BETA
confocal microscopy
Fluorescence Imaging
Infection

Software Mentioned

Excel
Mass Lynx
Mycromass
FluorCam
SigmaPlot

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