Temporal and spatial utilization of the alcohol dehydrogenase gene promoters during the development of Drosophila melanogaster

Developmental Biology
T J Lockett, M Ashburner

Abstract

The enzyme alcohol dehydrogenase of Drosophila melanogaster is encoded by a single structural gene (Adh) with two promoters, distal and proximal (PD and PP). During development these two promoters are used differently: the major Adh transcript of larvae is from PP, the major transcript of adult flies is from PD. At a few discrete times in development transcription occurs simultaneously from both promoters. In situ hybridization has been used to investigate the spatial and temporal aspects of promoter activity at these stages of development. Maternally inherited Adh transcripts are not localized in the embryo; they decay very rapidly after fertilization. Zygotic expression of Adh RNA begins after germ-band retraction, 10.5 hr after fertilization. Expression is confined to the fat body, but occurs from both distal and proximal promoters. By 15 hr expression is first seen in the gut, from PP. By the same time fat body expression from PD has ceased, and transcription in this tissue is exclusively from PP for the next 4 days. The steady-state level of Adh transcript begins to decline at the end of larval development. There is then the transient accumulation of transcripts from PD, but predominantly in the larval fat body, rather tha...Continue Reading

References

Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·P W Ingham
Jan 1, 1987·Annual Review of Genetics·U Schibler, F Sierra
Oct 11, 1985·Nucleic Acids Research·J A Fischer, T Maniatis
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·C Savakis, M Ashburner
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·J W PosakonyT Maniatis
Jan 1, 1987·Annual Review of Genetics·W Sofer, P F Martin
Jun 14, 1968·Annals of the New York Academy of Sciences·E H GrellJ B Murphy
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C Benyajati, J F Dray
Sep 1, 1970·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·Heinrich UrsprungNatalie Burroughs

❮ Previous
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Citations

Dec 24, 2010·Nature·Brenton R GraveleySusan E Celniker
Jun 26, 2001·Development, Growth & Differentiation·H Takata, T Kominami
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B P EnglandR Tjian
Mar 1, 1996·Insect Biochemistry and Molecular Biology·R J RussellJ G Oakeshott
Aug 1, 1995·Mutation Research·E W Vogel, A T Natarajan
Sep 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Venkateswara R ChintapalliJulian A T Dow
Mar 5, 2005·Journal of Morphology·Bernard MoussianChristiane Nüsslein-Volhard
Apr 30, 2003·Environmental and Molecular Mutagenesis·L AlvarezL M Sierra
Jan 17, 2012·Molecular Phylogenetics and Evolution·Crimgilt Mesías-GansbillerM Luz Pérez-Parallé
Sep 20, 2011·Journal of Insect Physiology·Fernando MartinEsther Alcorta
Dec 23, 2009·Journal of Biotechnology·Ijaz AliA Zahoor Swati
May 17, 2011·FEBS Letters·Haruna SakuraiKatsumi Kawasaki
Mar 25, 2008·Journal of Insect Physiology·David H DommerJohn B Phillips
Nov 6, 2007·Journal of Insect Physiology·Jodell E LinderDaniel E L Promislow
May 25, 2015·Insect Biochemistry and Molecular Biology·Gayle OverendJulian A T Dow
Apr 3, 2007·Developmental and Comparative Immunology·Stephanie M Brandt, David S Schneider
Feb 16, 2007·Gene Expression Patterns : GEP·Simon G SprecherVolker Hartenstein
Feb 14, 2007·Insect Biochemistry and Molecular Biology·Ivana Viktorinová, Ernst A Wimmer
Sep 4, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kouhei ShimajiMasamitsu Yamaguchi
Jul 17, 2004·The European Journal of Neuroscience·Tanja A GodenschwegeErich Buchner
Sep 4, 2004·Genes, Brain, and Behavior·M L SusterM B Sokolowski
May 22, 2009·The Journal of Comparative Neurology·Albert CardonaVolker Hartenstein
Nov 26, 2003·Journal of Evolutionary Biology·L Hadany, T Beker

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