Temporal changes of mRNA expression of matrix proteins and parathyroid hormone and parathyroid hormone-related protein (PTH/PTHrP) receptor in bone development

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
H KondoS Kasugai

Abstract

Expression of parathyroid hormone and parathyroid hormone-related protein (PTH/PTHrP) receptor is one of the osteoblastic phenotypes; however, it has not been clear whether this phenotype expression is a marker of immature or mature osteoblasts. We examined the temporal expression pattern of PTH/PTHrP receptor in bone development in vivo and in vitro compared with the expression of other osteoblastic phenotypes: osteopontin (OPN), bone sialoprotein (BSP) and osteocalcin (OC), alkaline phosphatase (ALP), and mineralization. Total RNA was extracted from rat calvariae, and cell culture of rat bone marrow at different developmental stages and then Northern blot hybridization were performed. Mineralization was detected with contact microradiography (CMR) in calvaria or with Alizarin Red S staining in bone marrow cell culture. Both in calvaria and in marrow cell culture, extensive expression of OPN, BSP, type I collagen (COL I), and ALP coincided with the onset of mineralization, and OC expression was observed after mineralized tissue formation. Notably, PTH/PTHrP receptor was expressed at an early developmental stage (prenatal day 14 in calvaria, day 5 in culture) when mineralized tissue was not formed and other osteoblastic phenoty...Continue Reading

References

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Citations

Jan 5, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T NesbittM K Drezner
Sep 1, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P C SchillerG A Howard
Sep 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hisatomo KondoF Richard Bringhurst
Jun 11, 2005·Bone·Hansjoerg Keller, Michaela Kneissel
Nov 2, 2006·Journal of Periodontal Research·S LossdörferA Jäger
Jun 24, 2005·Journal of Dental Research·S LossdörferA Jäger
Dec 3, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·M KorbasJ Ryczek
Aug 26, 2003·Journal of Cellular Biochemistry·Elizabeth H AllanT John Martin
Aug 22, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ken-ichi MiyamotoHideomi Yamagami
Mar 7, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Wagner R DuarteShohei Kasugai
Jul 30, 2010·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Hantang SunHideaki Suda
Nov 2, 2019·Cell Death & Disease·Min Kyung KimHong-Hee Kim
Jul 28, 2016·Stem Cells Translational Medicine·Saad Mechiche AlamiHalima Kerdjoudj
Mar 2, 1999·Biochemical and Biophysical Research Communications·W R DuarteS Kasugai

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