PMID: 8960978Oct 1, 1996Paper

Temporal characterization of microglia, IL-1 beta-like immunoreactivity and astrocytes in the dentate gyrus of hippocampal organotypic slice cultures

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
B W Coltman, C F Ide

Abstract

These studies demonstrate that murine hippocampal slice cultures possess neural-immune elements that show responses parallel to comparable in vivo models of neural-immune activation. Using immunocytochemical techniques, this study characterized the phenotypes of specific glial elements and the expression of the cytokine, interleukin-1 (IL-1 beta), in the hippocampal dentate gyrus over a period of 10 days in vitro (DIV). Preparation of organotypic slice cultures of neonatal mouse hippocampus produced cellular damage including axotomy of afferent fibers within the molecular layer of the dentate gyrus. This form of lesion-induced injury caused activation of neural-immune elements in the slice cultures. Staining with the microglial specific biotinylated Griffonia simplicifolia B4-isolectin revealed reactive microglia were most prevalent at 2 DIV and decreased in number from 4 to 10 DIV, whereas the initial population of resting microglia at 2 DIV increased approximately four-fold from 4 to 10 DIV. The presence of a round IL-1 beta-like immunophenotype closely paralleled the temporal and spatial distribution of the reactive form of microglia observed in the dentate gyrus. In addition, between 4 and 10 DIV, some IL-1 beta-like immuno...Continue Reading

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Citations

Nov 14, 1998·Brain Research. Molecular Brain Research·B Vernet-der GarabedianJ Mariani
Sep 19, 2008·Expert Opinion on Drug Metabolism & Toxicology·Gaylia Jean Harry, Andrew D Kraft
Mar 3, 2015·Frontiers in Neurology·Anna P MillerAleksandra Glavaski-Joksimovic
Mar 7, 2017·PloS One·Anna P MillerAleksandra Glavaski-Joksimovic
Jan 25, 2007·The Journal of Comparative Neurology·Elisabet BerglöfIngrid Strömberg
Apr 12, 2016·European Journal of Immunology·Johannes OrthgiessJens Eilers
Jul 13, 2018·Journal of Neuroinflammation·Daniela M MagalhãesCláudia A Valente
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Jun 22, 1999·Journal of Neuroscience Research·M Czapiga, C A Colton
Mar 10, 1999·Methods : a Companion to Methods in Enzymology·K Dobrenis

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