Temporal distinctions in the synthesis and accumulation of proteins by oocytes and cumulus cells during maturation in vitro of bovine oocytes

Molecular Reproduction and Development
B WuX Yang

Abstract

Successful in vitro maturation (IVM) of bovine oocytes requires continual and/or episodic protein synthesis by cumulus-oocyte complexes. This study was designed to expose time-dependent changes in protein synthesis and accumulation by bovine oocytes and cumulus cells during routine IVM. Silver staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated little if any change in protein species present or their relative contents in oocytes during IVM; one notable exception, however, was the gradual accumulation of a 39-kDa polypeptide between 4-24 hr of maturation culture. Cumulus cells, on the other hand, exhibited no qualitative differences during the period examined, but total protein content did increase during IVM. Metabolic labeling with [35S]-methionine, however, demonstrated changes in protein synthesis, both quantitative and qualitative, by both cell types. Oocytes exhibited a steady or slightly increasing rate of synthesis during the first 12 hr of IVM; thereafter, protein synthesis declined to about 10% of the initial rate by 40 hr in culture. In contrast, protein synthesis in cumulus cells was relatively constant during the first 24 hr. Of greater interest is the demonstration that ...Continue Reading

References

Jul 1, 1978·The Journal of Cell Biology·N B GilulaW H Beers
Feb 13, 1992·Nature·N YewG F Vande Woude
Jul 1, 1991·Molecular Reproduction and Development·P M KastropT A Kruip
Mar 1, 1991·Molecular Reproduction and Development·F de LoosT A Kruip
Sep 1, 1990·Journal of Reproduction and Fertility·P M KastropT A Kruip
Dec 1, 1990·Molecular Reproduction and Development·J Motlík, M Kubelka
Oct 1, 1990·Biology of Reproduction·R BuccioneJ J Eppig
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R S PaulesJ J Eppig
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
May 1, 1995·Molecular Reproduction and Development·L Rambhatla, K E Latham
May 1, 1995·Molecular Reproduction and Development·D J Nussbaum, R S Prather
Jun 1, 1995·The Journal of Experimental Zoology·H Tatemoto, T Terada
Jan 1, 1995·Molecular Reproduction and Development·A Hampl, J J Eppig
Mar 1, 1995·Molecular Reproduction and Development·L ZhangR A Godke
Jan 1, 1994·Reproduction, Nutrition, Development·J T Lévesque, M A Sirard
Oct 1, 1993·Acta Obstetricia Et Gynecologica Scandinavica·P V BaggerL Mortensen
Dec 1, 1993·Molecular Reproduction and Development·L GallV De Smedt
Jan 1, 1993·Molecular Reproduction and Development·X YangR H Foote

❮ Previous
Next ❯

Citations

Jan 28, 1998·Molecular Reproduction and Development·F GandolfiD T Armstrong
Mar 23, 2011·In Vitro Cellular & Developmental Biology. Animal·Gisele Zoccal MingotiJoaquim Mansano Garcia
Feb 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Jason R HerrickRebecca L Krisher
Jun 1, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Pilar CoyCarmen Matás
Sep 4, 2009·Molecular Reproduction and Development·Melissa Paczkowski, Rebecca Krisher
Oct 10, 2014·Biology of Reproduction·Francesca LolicatoBarend M Gadella

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.