Temporal dynamics of bacterial and fungal communities during the infection of Brassica rapa roots by the protist Plasmodiophora brassicae

PloS One
Lionel LebretonC Mougel

Abstract

The temporal dynamics of rhizosphere and root microbiota composition was compared between healthy and infected Chinese cabbage plants by the pathogen Plasmodiophora brassicae. When inoculated with P. brassicae, disease was measured at five sampling dates from early root hair infection to late gall development. The first symptoms of clubroot disease appeared 14 days after inoculation (DAI) and increased drastically between 14 and 35 DAI. The structure of microbial communities associated to rhizosphere soil and root from healthy and inoculated plants was characterized through high-throughput DNA sequencing of bacterial (16S) and fungal (18S) molecular markers and compared at each sampling date. In healthy plants, Proteobacteria and Bacteroidetes bacterial phyla dominated the rhizosphere and root microbiota of Chinese cabbage. Rhizosphere bacterial communities contained higher abundances of Actinobacteria and Firmicutes compared to the roots. Moreover, a drastic shift of fungal communities of healthy plants occurred between the two last sampling dates, especially in plant roots, where most of Ascomycota fungi dominated until they were replaced by a fungus assigned to the Chytridiomycota phylum. Parasitic invasion by P. brassicae d...Continue Reading

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Citations

Jun 26, 2020·Frontiers in Microbiology·Reid LongleyGregory Bonito
Sep 9, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Mónica M CollavinoO Mario Aguilar
Feb 19, 2020·BMC Plant Biology·Jose Manuel MartíCarlos P Garay
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Mar 20, 2021·Molecular Ecology·Kayleigh R O'KeeffeCharles E Mitchell
Apr 23, 2021·PLoS Pathogens·Bradley C Paasch, Sheng Yang He
Apr 27, 2021·Frontiers in Microbiology·Scott A KlasekLoïs Maignien
Dec 18, 2021·Frontiers in Microbiology·Ananda Y BandaraPaul D Esker

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Datasets Mentioned

BETA
PRJEB26948
ERS2513216
ERS2513353

Methods Mentioned

BETA
PCR

Software Mentioned

Bioconductor
vegan
R package “ Limma ”
custom
R script
PERL
R package “ vegan
lsmeans
R package “ Vegan ”
PIPE

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