Temporal embryonic transcription of chicken fast skeletal myosin heavy chain isoforms in the single comb white leghorn

Poultry Science
J GriffinM Wick

Abstract

There are numerous factors that can significantly influence embryonic development in poultry and thus make simple days of incubation (chronological age) a less than perfect metric for studying embryonic physiology. The developmental fast skeletal muscle myosin (MyHC), the predominant protein in the Pectoralis major (PM), is temporally expressed as a cadre of highly specific developmental isoforms. In the study described herein, a novel molecular technology (NanoString) was used to characterize the myosin isoform transcriptional patterns in the PM of Single Comb White Leghorn (SCWL) embryos. NanoString technology is based on quantitative analysis of the transcriptome through digital detection and quantification of target mRNA transcripts. Total RNA was isolated and gene transcription quantified using NanoString in embryonic muscle samples collected daily from 6 through 19 days of incubation. Data were analyzed using the LOESS smoothing function at a 95% confidence level. The temporal transcription of MyHC isoforms obtained in this study was consistent with the literature at higher specificity and resolution, thus validating NanoString for use in gene transcription analyses. The results support a hypothesis that the transcription...Continue Reading

References

Dec 1, 1986·The Journal of Cell Biology·J B Miller, F E Stockdale
Apr 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·W E TidymanE Bandman
Jan 14, 2004·Poultry Science·M WickM S Lilburn
Feb 19, 2008·Nature Biotechnology·Gary K GeissKrassen Dimitrov
May 10, 2011·BMC Biotechnology·Patricia P ReisSuzanne Kamel-Reid
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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