Temporal expression of nitric oxide synthase isoforms in healing Achilles tendon

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
J H LinG A Murrell

Abstract

We investigated the temporal expressions of the three nitric oxide synthase (NOS) isoforms by semi-quantitative polymerase chain reaction (PCR) assays and by immunoblot analysis, following Achilles tendon transection in rats. Four days after injury, there were increases in the steady-state levels of mRNA for all three NOS isoforms, with peaks for the inducible isoform (iNOS) (23-fold increase) at day 4, the endothelial isoform (eNOS) (24-fold increase) at day 7 and the neuronal isoform (bNOS) (seven-fold increase) at day 21. The temporal expression of NOS isoforms at a protein level was consistent with the results at the mRNA level. We have previously shown a five-fold increase in the NOS activity, as detected by 3H-arginine to 3H-citrulline conversion, at day 7 postinjury. These findings indicate that all three NOS isoforms are expressed during tendon healing with differential expression patterns during the various phases of tendon healing. These findings may prove clinically relevant with respect to strategies for regulating tendon healing.

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·V M VergeT Hökfelt
Jan 1, 1992·Neuron·D S Bredt, S H Snyder
Jul 1, 1992·Acta Physiologica Scandinavica·S Moncada
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
Feb 1, 1991·Trends in Neurosciences·J Garthwaite
Aug 1, 1991·European Journal of Clinical Investigation·S Moncada, E A Higgs
Jan 1, 1991·Journal of Clinical Gastroenterology·A TarnawskiH Gergely
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R G KnowlesS Moncada
Dec 19, 1989·European Journal of Pharmacology·J M PiqueJ V Esplugues
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·S J LeibovichA E Koch
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·K AsanoJ S Stamler
Dec 8, 1994·Nature·L KobzikJ S Stamler
Aug 1, 1995·American Journal of Respiratory Cell and Molecular Biology·P W ShaulR A Star
May 24, 1995·Biochimica Et Biophysica Acta·C RichterM Yaffee
Aug 3, 1993·European Journal of Pharmacology·S J KonturekJ Stachura
Nov 14, 1994·European Journal of Pharmacology·T IuvoneM Di Rosa
May 14, 1994·Lancet·E Anggård
Dec 30, 1993·The New England Journal of Medicine·T Y Wong, M C Mihm
Oct 1, 1995·The Histochemical Journal·J S PollockM Nakane
Jun 1, 1996·The Journal of Surgical Research·M R SchafferA Barbul
Apr 1, 1996·Bone·S J WimalawansaC Yallampalli
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·T J EvansJ Cohen
Jan 1, 1996·Life Sciences·N C StratmanV H Sethy
Jan 1, 1997·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·G A MurrellR F Warren
Jul 15, 1997·The Journal of Surgical Research·M R SchäfferA Barbul
Apr 16, 1998·The Journal of Clinical Investigation·K YamasakiT R Billiar
Jan 1, 1999·Journal of Clinical Gastroenterology·Y AkibaH Ishii
Jan 23, 1999·The American Journal of Pathology·F FacchettiR Badolato
Mar 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A D DiwanG A Murrell
Jul 1, 1996·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·M ShabaniD J Smith

❮ Previous
Next ❯

Citations

Jun 10, 2009·Cell and Tissue Research·Tatiana Carla TomiossoEdson Rosa Pimentel
Feb 10, 2007·British Journal of Sports Medicine·George A C Murrell
May 2, 2008·Journal of Orthopaedic Surgery and Research·Karsten Knobloch
Mar 30, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W ZhuG A Murrell
May 9, 2008·Clinical Orthopaedics and Related Research·George A C MurrellMin-Xia Wang
Mar 22, 2012·Advanced Drug Delivery Reviews·Scott P NicholsMark H Schoenfisch
Jan 17, 2012·Journal of Shoulder and Elbow Surgery·Ali R Bokhari, George A C Murrell
May 19, 2007·Journal of Shoulder and Elbow Surgery·George A C Murrell
Apr 4, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Andrija KrivicPredrag Sikiric
Sep 2, 2006·The Journal of Hand Surgery·Berish StrauchArthur A Pilla
Jan 26, 2017·Nature Reviews. Rheumatology·Neal L MillarIain B McInnes
Aug 16, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wei XiaYao Wang
Jan 20, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Z L SzomorG A C Murrell
Jan 26, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wei XiaGeorge A C Murrell
Oct 7, 2004·The Journal of Bone and Joint Surgery. American Volume·John A OgdenMarie E Marlow
Nov 26, 2019·Journal of Science and Medicine in Sport·Charlotte BrookesStuart M Raleigh
Jul 8, 2005·Journal of Biomechanics·James H-C Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
J H LinG A Murrell
Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
G A MurrellR F Warren
Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Wei XiaGeorge A C Murrell
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Wei XiaGeorge A C Murrell
© 2021 Meta ULC. All rights reserved