Temporal expression pattern of adhesion genes in human oral mucosal keratinocytes on type IV collagen-coated titanium

Journal of Biomedical Materials Research. Part a
Satoru TakeuchiTetsuhiko Tachikawa

Abstract

The cell adhesion extracellular matrix (ECM), and the cell adhesive system mediate various adhesion molecules. Cell adhesion regulates proliferation and differentiation. However, the cell adhesive systems between titanium and epithelial cells are still not clearly understood. In this study, we cultured human epithelial cells on type IV collagen-coated titanium (TiCol-4) and non-coated titanium (Ti). We used titanium disks of 30 mm diameter and 1.0 mm thickness. The titanium disks were first abraded using #800, #1200, #2400, and #4000 diamond pads and 0.3 mum alumina, and then washed with acetone, ethanol, and ultra-pure water in an ultrasonic cleaner for 10 min each. Human oral keratinocytes (hOMK) were seeded with 5.0 x 10(4) cells on TiCol-4 and Ti and placed in 6-well culture dishes. The cell adhesion examination was conducted with a Cell Coulter Counter after 1, 3, and 5 h. hOMK were observed using scanning electron microscopy (SEM). Real-time PCR was performed with four primers: gene laminin beta3, integrin beta4, integrin alpha1, and integrin alpha3. The results suggest that TiCol-4 could be used as a means for obtaining better hOMK than Ti. Type IV collagen could provide an excellent substratum for hOMK attachment on tit...Continue Reading

References

Mar 1, 1992·Journal of Periodontology·D BuserR C Williams
Mar 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R J MajeskaT A Einhorn
Jun 15, 1996·Journal of Cellular Biochemistry·R M Lafrenie, K M Yamada
Nov 1, 1996·Journal of Biochemistry·K NakazatoT Hayashi
Aug 1, 1997·Journal of Biomedical Materials Research·A El-GhannamI M Shapiro
Nov 14, 1997·The Journal of Biological Chemistry·J EmsleyR C Liddington
Feb 25, 1998·The Journal of Prosthetic Dentistry·H P Weber, D L Cochran
Jul 17, 1998·The Biological Bulletin·S E Baker, J C Jones
Jun 5, 1999·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·M McCracken
Feb 1, 2000·The Journal of Biological Chemistry·J KäpyläJ Heino
Jul 6, 2000·Journal of Biomedical Materials Research·U GeisslerK Wenzel
Aug 31, 2002·Biomolecular Engineering·P Linez-BataillonH F Hildebrand
Apr 25, 2003·Journal of Periodontology·Maristella Di CarmineAdriano Piattelli
Aug 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stefan RammeltAchim Biewener
Jun 1, 2005·Journal of Biomedical Materials Research. Part a·B BaharlooD M Brunette
May 25, 2006·Journal of Biomedical Materials Research. Part a·M MorraR Giardino
Aug 22, 2007·Journal of Materials Science. Materials in Medicine·H PaldanT O Närhi
Sep 27, 2007·Experimental Biology and Medicine·Valerie S LeBleuRaghu Kalluri
Sep 24, 2008·Experimental Cell Research·Tristin D AbairRoy Zent

❮ Previous
Next ❯

Citations

Feb 2, 2012·Journal of Tissue Engineering and Regenerative Medicine·Shingo KawabataYasuhiko Tabata
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yi ZhuJiandong Ding

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.