Temporal gene expression during differentiation of human embryonic stem cells and embryoid bodies

Human Reproduction
Tamar DvashRachel Eiges

Abstract

The aim of this study was to characterize human embryonic stem (ES) cells at the molecular level by performing large-scale complementary DNA (cDNA) analysis using DNA micro-arrays. The transcription profile of human ES cells was determined by comparing it to 2, 10 and 30-day old embryoid bodies (EBs) using Affymetrix Genechip human micro-arrays (U133). According to this analysis we demonstrate that two human ES cell lines are more close to each other than to their differentiated derivatives. We also show the spectrum of cytokine receptors that they express, and demonstrate the presence of five genes that are highly specific to human ES cells and to germ cells. Moreover, by profiling different stages in the differentiation of human embryoid bodies, we illustrate the clustering of five sets of temporally expressed genes, which could be related to the sequential stages of embryonic development. Among them are known genes that are involved in early pattern formation. The present study provides a molecular basis for the identity of human ES cells and demonstrates that during their in vitro differentiation they express embryonic specific genes in a stage specific manner.

References

Nov 6, 1998·Science·J A ThomsonJ M Jones
Nov 14, 1998·Biochimica Et Biophysica Acta·E C Crouch
Dec 28, 1999·Reproduction, Fertility, and Development·T A PeltonP D Rathjen
Apr 5, 2000·Nature Biotechnology·B E ReubinoffA Bongso
Sep 27, 2000·Biochimica Et Biophysica Acta·J R Gillespie, V N Uversky
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SchuldinerN Benvenisty
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·D S KaufmanJ A Thomson
Sep 11, 2001·Brain Research·M SchuldinerN Benvenisty
Nov 22, 2001·Experimental Neurology·M K CarpenterM S Rao
Dec 4, 2001·Nature Biotechnology·B E ReubinoffT Ben-Hur
Feb 12, 2002·Nature Reviews. Genetics·Hiroshi HamadaYukio Saijoh
May 30, 2002·Journal of Anatomy·C MummeryM Pera
Sep 14, 2002·Science·Natalia B IvanovaIhor R Lemischka
Oct 2, 2002·FEBS Letters·Rachel Eiges, Nissim Benvenisty
Aug 19, 2003·Developmental Biology·Noboru SatoAli H Brivanlou
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jamie M SpergerJames A Thomson
Dec 23, 2003·Stem Cells·Mark RichardsAriff Bongso

❮ Previous
Next ❯

Citations

Feb 27, 2010·In Vitro Cellular & Developmental Biology. Animal·Tsvia FrumkinDalit Ben-Yosef
Jul 9, 2005·Nature Biotechnology·Peter W AndrewsUNKNOWN Steering Committee of the International Stem Cell Initiative
Jun 9, 2005·Nature Biotechnology·Lisa M Hoffman, Melissa K Carpenter
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Natanel Korin, Shulamit Levenberg
Aug 12, 2010·Cellular Reprogramming·Charles A EasleyGerald P Schatten
Sep 19, 2006·Stem Cells and Development·Steven Shoei-Lung LiSher Singh
Sep 25, 2008·Stem Cells and Development·Suzanne D WestfallToshihiko Ezashi
Jun 3, 2009·The Journal of Clinical Investigation·Hervé AcloqueM Angela Nieto
May 27, 2008·BMC Developmental Biology·Paolo CinelliKurt Bürki
Dec 29, 2007·BMC Genomics·Thomas C SchulzXianmin Zeng
Mar 31, 2007·BMC Genomics·Kagnew Hailesellasse SeneMiguel A Andrade-Navarro
Jul 26, 2006·Pediatric Research·Tamar DvashRachel Eiges
Jan 31, 2013·Development·Zengrong Zhu, Danwei Huangfu
Mar 13, 2008·PloS One·Chee Gee LiewPeter W Andrews
Jul 14, 2011·Regenerative Medicine·Juan-Carlos Biancotti, Nissim Benvenisty
Jul 8, 2009·Pakistan Journal of Biological Sciences : PJBS·Mahmoud Hashemi-TabarFereshteh Negad Dehbashi
Jun 28, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Mi-Young SonYong-Mahn Han
May 1, 2007·Regenerative Medicine·Henia Darr, Nissim Benvenisty
Mar 7, 2006·FEBS Letters·Petr DvorakAles Hampl
Apr 3, 2009·Biologicals : Journal of the International Association of Biological Standardization·J QinZ M Cai
Jan 9, 2008·Molecular and Cellular Endocrinology·D Ben-YosefR Eiges
Sep 1, 2007·Experimental Hematology·Dong ChenChristina Kendziorski
Jul 25, 2007·Current Opinion in Chemical Biology·Lawrence W Stanton, Manjiri M Bakre
Jul 5, 2007·Journal of Bioscience and Bioengineering·Hiroshi Kurosawa
Feb 17, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Henrik Semb
Feb 7, 2009·Biotechnology Progress·Andrés M Bratt-LealTodd C McDevitt
Aug 30, 2008·British Journal of Pharmacology·A VaranouS L Minger
May 27, 2006·Stem Cells·Siamak Tabibzadeh, Ali Hemmati-Brivanlou
Oct 29, 2008·Journal of Tissue Engineering and Regenerative Medicine·Joseph M MessanaZijun Zhang
Oct 14, 2006·Stem Cells·Tamar DvashNissim Benvenisty
Sep 15, 2007·Neuropathology and Applied Neurobiology·Y A ChristouP N Monk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Jamie M SpergerJames A Thomson
Proceedings of the National Academy of Sciences of the United States of America
Maya SchuldinerNissim Benvenisty
© 2021 Meta ULC. All rights reserved