Temporal instability of the post-surgical maxillary sinus microbiota

BMC Infectious Diseases
Ioannis KoutsourelakisAndrew P Lane

Abstract

Chronic rhinosinusitis is an inflammatory disorder in which the role of bacteria remains uncertain. While sinus outflow obstruction is often an initiating event, mucosal inflammation and dysbiosis may persist or develop in sinuses with widely patent surgical openings. Understanding of the relationship between dysbiosis and chronic sinus inflammation is obfuscated by inter-individual microbiota variability and likely intra-individual temporal variation that has yet to be defined. In this study, long-term microbiota stability is investigated within surgically-opened maxillary sinuses of individuals with and without sinus inflammatory disease. Maxillary sinus swabs were performed in 35 subjects with longstanding maxillary antrostomies. Subjects with and without active chronic maxillary sinusitis were included. Repeat swabs were obtained from the same sinuses after a prolonged interval (mean 719 ± 383 days). Patients were categorized based on the inflammatory status of the sinus mucosa at times of sample collection, as assessed by nasal endoscopy. Total DNA from swab eluents was extracted, and the microbiota characterized using 16S rRNA gene sequencing followed by taxonomic classification. Prevalence and abundance of genera were de...Continue Reading

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Citations

Mar 13, 2019·Current Allergy and Asthma Reports·Thad W VickeryJeffrey D Suh
Aug 30, 2020·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Weigang GanJunming Xian
Nov 26, 2020·International Forum of Allergy & Rhinology·Richard R OrlandiBing Zhou

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Methods Mentioned

BETA
PCR

Software Mentioned

QIIME
metagenomeSeq
PANDAseq
R
Vegan
UCHIME
Phyloseq
ANOSIM
Quantitative Insights Into Microbial Ecology ( QIIME
ggplot218

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