Dec 14, 2011

Temporal pattern of expression and colocalization of microglia/macrophage phenotype markers following brain ischemic injury in mice

Journal of Neuroinflammation
Carlo PeregoMaria-Grazia De Simoni

Abstract

Emerging evidence indicates that, similarly to what happens for peripheral macrophages, microglia can express different phenotypes depending on microenvironmental signals. In spite of the large literature on inflammation after ischemia, information on M/M phenotype marker expression, their colocalization and temporal evolution in the injured brain is lacking. The present study investigates the presence of microglia/macrophage phenotype markers, their temporal expression, whether they are concomitantly expressed by the same subpopulation, or they are expressed at distinct phases or locations in relation to the ischemic lesion. Volume of ischemic lesion, neuronal counts and TUNEL staining were assessed in C57Bl/6 mice at 6-12-24-48 h and 7d after permanent occlusion of the middle cerebral artery. At the same time points, the expression, distribution in the lesioned area, association with a definite morphology and coexpression of the microglia/macrophage markers CD11b, CD45, CD68, Ym1, CD206 were assessed by immunostaining and confocal microscopy. The results show that: 1) the ischemic lesion induces the expression of selected microglia/macrophage markers that develop over time, each with a specific pattern; 2) each marker has a g...Continue Reading

  • References49
  • Citations130

References

  • References49
  • Citations130

Citations

Mentioned in this Paper

Macrophage-1 Antigen
Microorganism
Ischemia
Brain Injuries
Mild Traumatic Brain Injury
Insulin-Like Growth Factor I
Salicylhydroxamic acid
Cortex Bone Disorders
Heparin
Immunoreactivity

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