Temporal profile of thrombogenesis in the cerebral microcirculation after traumatic brain injury in mice

Journal of Neurotrauma
Susanne M SchwarzmaierNikolaus Plesnila

Abstract

Traumatic brain injury (TBI) is associated with an almost immediate reduction in cerebral blood flow (CBF). Because cerebral perfusion pressure is often normal under these circumstances it was hypothesized that the reduction of post-traumatic CBF has to occur at the level of the microcirculation. The aim of the current study was to investigate whether cerebral microvessels are involved in the development of blood flow disturbances following experimental TBI. C57/BL6 mice (n = 12) were intubated and ventilated under control of end-tidal Pco(2) ((ET)P(CO2)). After preparation of a cranial window and baseline recordings, the animals were subjected to experimental TBI by controlled cortical impact (CCI; 6 m/sec, 0.5 mm). Vessel lumina and intravascular cells were visualized by in vivo fluorescence microscopy (IVM) using the fluorescent dyes FITC-dextran and rhodamine 6G, respectively. Vessel diameter, cell-endothelial interactions, and thrombus formation were quantified within the traumatic penumbra by IVM up to 2 h after CCI. Arteriolar diameters increased after CCI by 26.2 +/- 2.5% (mean +/- SEM, p < 0.01 versus baseline), and remained at this level until the end of the observation period. Rolling of leukocytes on the cerebrovasc...Continue Reading

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Methods Mentioned

BETA
fluorescence microscopy
fluorescence
transgenic

Related Concepts

Structure of Arteriole
Brain
Mild Traumatic Brain Injury
Cell Adhesion
Structure of Cerebral Artery
Cerebrovascular Circulation
Fluorochromes
Mice, Inbred C57BL
Microcirculation
Immunofluorescence Microscopy

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