Abstract
Cytokines drive many inflammatory diseases, including asthma. Understanding the molecular mechanisms responsible for cytokine secretion will allow us to develop novel strategies to repress inflammation in the future. Harnessing the power of endogenous anti-inflammatory proteins is one such strategy. In this study, we investigate the p38 MAPK-mediated regulatory interaction of two anti-inflammatory proteins, mitogen-activated protein kinase phosphatase 1 (MKP-1) and tristetraprolin (TTP), in the context of asthmatic inflammation. Using primary cultures of airway smooth muscle cells in vitro, we explored the temporal regulation of IL-6 cytokine mRNA expression upon stimulation with TNF-α. Intriguingly, the temporal profile of mRNA expression was biphasic. This was not due to COX-2-derived prostanoid upregulation, increased expression of NLRP3 inflammasome components, or upregulation of the cognate receptor for TNF-α-TNFR1. Rather, the biphasic nature of TNF-α-induced IL-6 mRNA expression was regulated temporally by the RNA-destabilizing molecule, TTP. Importantly, TTP function is controlled by p38 MAPK, and our study reveals that its expression in airway smooth muscle cells is p38 MAPK-dependent and its anti-inflammatory activity...Continue Reading
References
Feb 1, 1995·Pulmonary Pharmacology·P R JohnsonJ L Black
May 1, 1996·Immunity·G A TaylorP J Blackshear
Mar 1, 1997·British Journal of Pharmacology·M G BelvisiJ A Mitchell
Jun 1, 1997·British Journal of Pharmacology·L Pang, A J Knox
Aug 14, 1998·Science·E CarballoP J Blackshear
Dec 10, 1998·British Journal of Pharmacology·M G BelvisiJ A Mitchell
Dec 24, 1998·British Journal of Pharmacology·L PangA J Knox
May 18, 1999·Molecular and Cellular Biology·W S LaiP J Blackshear
Jun 29, 2000·American Journal of Respiratory Cell and Molecular Biology·S McKayH S Sharma
Dec 6, 2000·American Journal of Respiratory Cell and Molecular Biology·A J AmmitR A Panettieri
Sep 5, 2001·Molecular and Cellular Biology·K R MahtaniA R Clark
Sep 15, 2005·The Journal of Allergy and Clinical Immunology·Aili L Lazaar, Reynold A Panettieri
Oct 13, 2006·Molecular and Cellular Biology·Wi S LaiPerry J Blackshear
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Razao IssaKian Fan Chung
Mar 4, 2008·American Journal of Respiratory Cell and Molecular Biology·Timo QuanteAlaina J Ammit
May 7, 2009·FEBS Letters·Corina TudorJonathan L E Dean
May 14, 2009·The Journal of Pharmacology and Experimental Therapeutics·Elizabeth M KingRobert Newton
Jan 2, 2010·Biochimica Et Biophysica Acta·John P MoutzourisAlaina J Ammit
Dec 28, 2011·Cellular Signalling·Melanie ManetschAlaina J Ammit
Feb 11, 2012·Current Medicinal Chemistry·M R DoddareddyA J Ammit
Mar 1, 2012·British Journal of Pharmacology·M ManetschA J Ammit
Jun 30, 2012·Biochimica Et Biophysica Acta·Wenchi CheAlaina J Ammit
Feb 23, 2013·Biochimica Et Biophysica Acta·Seth A Brooks, Perry J Blackshear
Mar 28, 2013·PloS One·Melanie ManetschAlaina J Ammit
Apr 26, 2013·American Journal of Respiratory Cell and Molecular Biology·Jeremy A HirotaAlaina J Ammit
Oct 15, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H Im, A J Ammit
Mar 22, 2014·PloS One·Md Mostafizur RahmanAlaina J Ammit
May 28, 2014·Allergy·H AlkhouriA J Ammit
Nov 28, 2014·Molecular Pharmacology·Pavan Prabhala, Alaina J Ammit
Citations
Jan 29, 2016·Cellular Signalling·Md Mostafizur RahmanAlaina J Ammit
Nov 18, 2015·American Journal of Respiratory Cell and Molecular Biology·Brijeshkumar S PatelAlaina J Ammit
Jan 31, 2016·Journal of Cellular Physiology·Pavan PrabhalaAlaina J Ammit
Dec 21, 2016·American Journal of Respiratory Cell and Molecular Biology·Brijeshkumar S PatelAlaina J Ammit
Mar 12, 2017·The Journal of Biological Chemistry·Robert NewtonAntony N Gerber
Mar 31, 2018·Cancer Medicine·Kathrin GärtnerReinhard Zeidler
Nov 11, 2017·Medical Oncology·Jian GuoJiazeng Xia
Mar 11, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Peta BradburyAlaina J Ammit
Oct 16, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y S Prakash
Aug 12, 2017·Respiratory Research·Seyed M MoosaviAlaina J Ammit
Oct 19, 2019·Respiratory Research·Cassandra P NaderAlaina J Ammit
May 26, 2020·BMC Cardiovascular Disorders·Jingjing JiJinghua Liu
Jan 1, 2017·Journal of Biochemistry·Akira Fukao, Toshinobu Fujiwara
Nov 8, 2015·The Journal of Biological Chemistry·Suharsh ShahRobert Newton
Feb 9, 2017·Nucleic Acids Research·Kishan K NyatiTadamitsu Kishimoto
Sep 29, 2020·Frontiers in Immunology·Ishita ChoudharySonika Patial
Jul 1, 2017·Scientific Reports·Tina TangAndrew R Clark
Dec 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hiroaki TakemotoYoshinori Masuo
Jul 24, 2021·Cellular and Molecular Neurobiology·Jaedeok KwonBrian J Morris
Sep 14, 2021·Frontiers in Physiology·Li LiangQing Yun Li