PMID: 6165834May 1, 1981Paper

Temporal regulation of human cytomegalovirus transcription at immediate early and early times after infection

Journal of Virology
M W WathenM F Stinski

Abstract

The immediate early transcripts of human cytomegalovirus originated from restricted regions of the viral genome. In contrast, transcription at early times was complementary to all regions of the viral genome that were fractionated by restriction endonuclease treatment followed by agarose gel electrophoresis. The viral genome was also extensively transcribed when 2 h of protein synthesis or longer was permitted after infection in permissive cells treated with an inhibitor of viral DNA replication or in nonpermissive cells of animal origin that permit little or no viral DNA replication. The size and in vitro translation products of the cytomegalovirus-specified mRNA's at immediate early and early times after infection were determined. Discrete size classes of virus-specified polyadenylated RNA accumulated on the polyribosomes of cells infected in the presence of an inhibitor of protein synthesis. When 2 or 24 h of protein synthesis occurred after infection, there were changes in the relative abundance of the virus-specified RNAs that accumulated on polyribosomes. Treatment of nonpermissive cells had little effect on the size classes of viral RNA found associated with the polyribosomes at early times after infection. These viral m...Continue Reading

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Citations

Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·W A Bresnahan, T E Shenk
Mar 20, 2013·Acta Biochimica Et Biophysica Sinica·Kezhen WangTianhong Zhou
Jan 1, 2000·Archives of Pharmacal Research·J J HyunH J Kim
Mar 2, 2012·Microbiology and Immunology·Yejin KwonChang-Yong Cha
Sep 1, 1992·American Journal of Respiratory Cell and Molecular Biology·M M MonickG W Hunninghake
Oct 17, 1998·Methods : a Companion to Methods in Enzymology·C Söderberg-NauclérJ A Nelson
Jan 1, 1984·Journal of Virology·R M StenbergM F Stinski
Feb 1, 1984·Journal of Virology·G JahnB Fleckenstein

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