Temporal sequence of interleukin 1 alpha-mediated stimulation and inhibition of bone formation by isolated fetal rat calvaria cells in vitro

Cytokine
L G Ellies, J E Aubin

Abstract

Cytokines released at sites of inflammation and infection may alter normal bone remodeling processes resulting in pathologic bone destruction or bone formation. Interleukin 1, an inflammatory mediator, has been shown to stimulate as well as inhibit parameters associated with bone formation. In this study we have examined temporal aspects of the biphasic effects of recombinant interleukin 1 alpha (IL 1 alpha) on the differentiation of osteoprogenitor cells into bone-forming osteoblasts (bone nodules) in vitro. A dose-dependent stimulation of bone formation over a concentration range of 0.5 to 50 U/mL (1.4 x 10(-12) to 1.4 x 10(-10) M) was observed when preconfluent, primary cultures of fetal rat calvaria (RC) cells were pulsed with IL 1 alpha for 72 to 96 hr from the beginning of the culture period. This was correlated with a stimulation of cell proliferation and alkaline phosphatase activity measured during the late log phase of growth. In contrast, continuous exposure to IL 1 alpha or exposure to IL 1 alpha after confluency resulted in inhibition of bone nodule formation and alkaline phosphatase activity. IL 1 alpha-stimulated prostaglandin E2 (PGE2) production until the RC cells became multilayered, but the addition of the cy...Continue Reading

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Citations

Aug 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L G ElliesJ E Aubin
Feb 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A Herbertson, J E Aubin
Jul 14, 1995·Mechanisms of Ageing and Development·L Rabadjija, P Goldhaber
Jun 28, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Edwin LamersJohn A Jansen
Apr 25, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Dominic Falconi, Jane E Aubin
Jan 29, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Fu-Hsiumg LinBrian E Brigman
Sep 8, 2004·Life Sciences·Natsuko TanabeKoichi Ito
May 12, 2006·Periodontology 2000·Francis J HughesGianluca Martuscelli
Apr 1, 1997·Acta Orthopaedica Scandinavica·A FrostO Ljunggren
Nov 16, 1992·Biochemical and Biophysical Research Communications·L G ElliesJ E Aubin
Feb 23, 2018·Journal of Biomedical Materials Research. Part a·Laura Datko WilliamsDelphine Dean
Jul 19, 2005·Orthodontics & Craniofacial Research·J GlowackiS Mizuno
Mar 27, 2001·Equine Veterinary Journal·B von RechenbergJ A Auer

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