Temporal SILAC-based quantitative proteomics identifies host factors involved in chikungunya virus replication

Proteomics
Emmely E TreffersPeter A van Veelen

Abstract

Chikungunya virus (CHIKV) is an arthropod-borne reemerging human pathogen that generally causes a severe persisting arthritis. Since 2005, the virus has infected millions of people during outbreaks in Africa, Indian Ocean Islands, Asia, and South/Central America. Many steps of the replication and expression of CHIKV's 12-kb RNA genome are highly dependent on cellular factors, which thus constitute potential therapeutic targets. SILAC and LC-MS/MS were used to define the temporal dynamics of the cellular response to infection. Using samples harvested at 8, 10, and 12 h postinfection, over 4700 proteins were identified and per time point 2800-3500 proteins could be quantified in both biological replicates. At 8, 10, and 12 h postinfection, 13, 38, and 106 proteins, respectively, were differentially expressed. The majority of these proteins showed decreased abundance. Most subunits of the RNA polymerase II complex were progressively degraded, which likely contributes to the transcriptional host shut-off observed during CHIKV infection. Overexpression of four proteins that were significantly downregulated (Rho family GTPase 3 (Rnd3), DEAD box helicase 56 (DDX56), polo-like kinase 1 (Plk1), and ubiquitin-conjugating enzyme E2C (UbcH...Continue Reading

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Citations

Jun 15, 2016·Viruses·Jelke J Fros, Gorben P Pijlman
Nov 9, 2016·Virology·Colleen R Reid, Tom C Hobman
Sep 16, 2020·Antimicrobial Agents and Chemotherapy·Kristina Kovacikova, Martijn J van Hemert
Dec 14, 2016·Journal of Proteome Research·Hongxing ZhaoSara Bergström Lind
Jun 16, 2021·Annual Review of Virology·Alice Fletcher-Etherington, Michael P Weekes

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