Abstract
Identifying developmental proteins could lead to markers of bladder progenitor cells, which could be used to investigate bladder diseases. We recently reported a novel embryonic stem cell model in which to study differential protein expression patterns during bladder development. Differential and temporal expressions of the endodermal proteins known as forkhead box (Foxa1 and Foxa2) were observed. In the current study we further delineated these protein expression patterns. Epithelium was removed from the underlying mesenchyma from embryonic day 18 rat bladders. Heterospecific recombinant xenografts were created by combining embryonic stem cells plus embryonic bladder mesenchyma and placed beneath the renal capsule of mouse hosts. Grafts were harvested at 16, 18, 21, 28, 35 and 42 days, and evaluated with hematoxylin and eosin, trichrome staining, and immunohistochemistry for uroplakin, smooth muscle alpha-actin, p63, Foxa1, Foxa2 and androgen receptor. At 16 days uroplakin was detectable and it seemed to correlate with the loss of Foxa2, while Foxa1 remained at all time points. Androgen receptor was first noted in stroma at day 16. It localized to urothelial nuclei at day 21 and was undetectable at 42 days. Adjacent to the uro...Continue Reading
References
Jan 1, 1986·The Prostate·Y SugimuraR M Bigsby
Jun 1, 1993·Endocrinology·A A Donjacour, G R Cunha
Jan 1, 1996·Acta Anatomica·L S BaskinG R Cunha
Jan 1, 1997·European Urology·S ImadaT Takenaka
Jun 1, 1997·The Journal of Urology·L S BaskinG R Cunha
Oct 20, 2000·In Vitro Cellular & Developmental Biology. Animal·W LiuL Baskin
Mar 21, 2001·The Journal of Urology·L BaskinG Cunha
Jul 18, 2001·The Journal of Urology·S SalmiS Mäkelä
Sep 25, 2004·The Prostate·Janni MirosevichRobert J Matusik
Mar 23, 2005·Archives of Histology and Cytology·Gerald R CunhaTakeshi Kurita
May 20, 2005·Differentiation; Research in Biological Diversity·Andrea StaackGerald R Cunha
Apr 25, 2006·Lancet·Anthony AtalaAlan B Retik
Jan 5, 2007·Development·Ryuma HaraguchiGen Yamada
Feb 10, 2007·Developmental Biology·Siam OottamasathienRobert J Matusik
Apr 5, 2007·Journal of the National Cancer Institute·Hiroshi MiyamotoChawnshang Chang
May 11, 2007·Differentiation; Research in Biological Diversity·Yoshiyuki ShiroyanagiLaurence S Baskin
Jul 11, 2007·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Martina PrelogLothar Bernd Zimmerhackl
Sep 18, 2007·The Journal of Urology·Patrick W MufarrijMichael D Stifelman
Citations
May 17, 2012·PloS One·David J DeGraffRobert J Matusik
Sep 16, 2011·Regenerative Medicine·Arun K Sharma
Sep 20, 2011·Urologic Oncology·David J DeGraffRosalyn M Adam
Dec 8, 2009·Urology·Richard N Yu, Carlos R Estrada
Sep 8, 2011·BJU International·Felix WezelDavid F M Thomas
Apr 19, 2013·International Journal of Urology : Official Journal of the Japanese Urological Association·Yasuhiro Sumino, Hiromitsu Mimata
Feb 18, 2014·Differentiation; Research in Biological Diversity·Renea A Taylor, Gail P Risbridger
Aug 1, 2015·Endocrine-related Cancer·Alan P Lombard, Maria Mudryj
Nov 5, 2016·Tissue Engineering. Part B, Reviews·Petra de GraafLaetitia M O de Kort
Nov 30, 2016·Nature Reviews. Urology·Hironobu YamashitaDavid J DeGraff
Mar 25, 2014·Stem Cells Translational Medicine·Stephanie L OsbornEric A Kurzrock
Jun 28, 2017·Molecular and Cellular Endocrinology·Satoshi InoueHiroshi Miyamoto
Nov 6, 2009·Nature Reviews. Urology·Karen J Aitken, Darius J Bägli
May 24, 2019·Human Cell·Dorota GilPiotr Laidler
Jun 17, 2011·Tsitologiia·B K PopovN S Petrov
Mar 18, 2020·Journal of Clinical Medicine·Ahmed Abdal DayemSsang-Goo Cho
Sep 27, 2019·Scientific Reports·Yuta InoueOsam Mazda
Mar 1, 2017·Cancers·Peng LiHiroshi Miyamoto
Oct 2, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yongjun QuanXiaodong Zhang