Temporal Stability of the Salivary Microbiota in Oral Health

PloS One
D BelstrømB J Paster

Abstract

Saliva is a biological fluid suitable for biomarker analysis, and differences in the salivary microbiota in oral health and disease have been reported. For such comparative analyses, time of sampling is critical since the bacterial composition may vary throughout the day, i.e., diurnal variation. The purpose of this study is to compare the salivary microbiome over time to determine the optimal time for sampling. Stimulated saliva samples were collected from 5 orally healthy individuals in 4 h intervals for 24 h, and collection was repeated 7 days later (number of samples per person, n = 12, total number of samples, n = 60). Salivary microbiota was analyzed using the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS), and statistical analysis was performed using the Kruskal-Wallis test with Benjamini-Hochberg's correction for multiple comparisons, cluster analysis, principal component analysis and correspondence analysis. From a total of 60 saliva samples, 477 probe targets were collectively identified with a mean number of probes per sample of 207 (range: 153-307). Little or no variation in microbial profiles within subjects was observed over time. Although there was considerable variation between subj...Continue Reading

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Citations

Jan 17, 2017·Trends in Microbiology·Jonathon L BakerXuesong He
Mar 19, 2016·Journal of Oral Microbiology·Daniel BelstrømBruce J Paster
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Software Mentioned

MeV
HOMI
ProbeSeq for HOMI NGS
Probeseq
HOMIM
BLAST
GraphPad prism

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