PMID: 9525589Apr 3, 1998Paper

Temporal usage of multiple promoters during the life cycle of human papillomavirus type 31b

Journal of Virology
M A Ozbun, C Meyers

Abstract

The life cycles of human papillomaviruses (HPVs) are dependent upon the differentiation of the epithelial cells they infect. HPV type 31b (HPV31b) virions can be purified following the growth of a latently HPV-infected cell line (CIN-612 9E) in the organotypic or raft system. Treatment of the CIN-612 9E raft tissues with protein kinase C (PKC) activators is required for upregulation of late gene expression and efficient production of virions. We employed the raft culture system to study the temporal usage of HPV31b promoters during the viral life cycle. We compared monolayer cultures of CIN-612 9E cells, untreated CIN-612 9E raft tissues, and PKC-induced CIN-612 9E raft tissues harvested at various time points during epithelial differentiation. We found that the HPV31b major early promoter precisely maps to nucleotide (nt) 99 (P99). A transcriptional start site for both early and late gene transcripts mapped upstream of P99 at nt 77 (P77). The P77 and P99 promoters were used constitutively throughout the HPV31b life cycle; however, initiation from P99 was much stronger than from P77. Mapping of the differentiation-induced P742 promoter revealed multiple start sites. These start sites were difficult to detect in monolayer cultur...Continue Reading

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Citations

Mar 10, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·S. A. Southern, C. S. Herrington
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Michelle S Longworth, Laimonis A Laimins
Feb 26, 2005·Journal of Virology·Jason M Bodily, Craig Meyers
Aug 30, 2008·Journal of Virology·Michael J LaceThomas H Haugen
Jun 24, 2020·Pathogens·Michelle Mac, Cary A Moody
Sep 5, 2002·Journal of Virology·Jennifer L Bromberg-White, Craig Meyers
Apr 23, 2002·Journal of Virology·Craig MeyersKathrin U Jansen
May 2, 2009·Journal of Dental Research·M J Conway, C Meyers
Mar 26, 2014·Transboundary and Emerging Diseases·F BocanetiG Borzacchiello
Mar 27, 2012·Journal of the National Cancer Institute·Lisa MirabelloRobert D Burk
Sep 17, 2020·Viruses·Jón Pol GalesMaria Dimitrova
Oct 26, 2011·Reviews in Medical Virology·Kong-Nan Zhao, Jiezhong Chen
Jan 11, 2020·Seminars in Immunopathology·Megan BurleyJoanna L Parish
Nov 24, 1999·The Journal of General Virology·T H BraunsteinB Norrild
Nov 26, 2013·Future Microbiology·Shiyuan Hong, Laimonis A Laimins
Oct 13, 2006·The Journal of General Virology·Christy M HebnerLaimonis A Laimins

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