Temporal Variability of Oral Microbiota over 10 Months and the Implications for Future Epidemiologic Studies

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
Emily VogtmannChristian C Abnet

Abstract

Background: Few studies have prospectively evaluated the association between oral microbiota and health outcomes. Precise estimates of the intrasubject microbial metric stability will allow better study planning. Therefore, we conducted a study to evaluate the temporal variability of oral microbiota.Methods: Forty individuals provided six oral samples using the OMNIgene ORAL kit and Scope mouthwash oral rinses approximately every two months over 10 months. DNA was extracted using the QIAsymphony and the V4 region of the 16S rRNA gene was amplified and sequenced using the MiSeq. To estimate temporal variation, we calculated intraclass correlation coefficients (ICCs) for a variety of metrics and examined stability after clustering samples into distinct community types using Dirichlet multinomial models (DMMs).Results: The ICCs for the alpha diversity measures were high, including for number of observed bacterial species [0.74; 95% confidence interval (CI): 0.65-0.82 and 0.79; 95% CI: 0.75-0.94] from OMNIgene ORAL and Scope mouthwash, respectively. The ICCs for the relative abundance of the top four phyla and beta diversity matrices were lower. Three clusters provided the best model fit for the DMM from the OMNIgene ORAL samples, ...Continue Reading

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Citations

Nov 27, 2019·Journal of the American Heart Association·Charlene E GohRyan T Demmer
Nov 24, 2020·Periodontology 2000·Flavia TelesJulie T Marchesan
Jan 30, 2021·Clinical and Translational Gastroenterology·Medini K AnnavajhalaJulian A Abrams
Aug 28, 2021·Microorganisms·Yukiko YanoChristian C Abnet

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