Temporal variations in bacterial community diversity and composition throughout intensive care unit renovations.

Microbiome
Jessica ChopykDavid T Pride

Abstract

Inanimate surfaces within a hospital serve as a reservoir of microbial life that may colonize patients and ultimately result in healthcare associated infections (HAIs). Critically ill patients in intensive care units (ICUs) are particularly vulnerable to HAIs. Little is known about how the microbiome of the ICU is established or what factors influence its evolution over time. A unique opportunity to bridge the knowledge gap into how the ICU microbiome evolves emerged in our health system, where we were able to characterize microbial communities in an established hospital ICU prior to closing for renovations, during renovations, and then after re-opening. We collected swab specimens from ICU bedrails, computer keyboards, and sinks longitudinally at each renovation stage, and analyzed the bacterial compositions on these surfaces by 16S rRNA gene sequencing. Specimens collected before ICU closure had the greatest alpha diversity, while specimens collected after the ICU had been closed for over 300 days had the least. We sampled the ICU during the 45 days after re-opening; however, within that time frame, the alpha diversity never reached pre-closure levels. There were clear and significant differences in microbiota compositions at...Continue Reading

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Citations

Mar 9, 2021·Computational and Structural Biotechnology Journal·Vanessa N KavamuraTim H Mauchline
Dec 17, 2021·The Science of the Total Environment·Kexin LiSongnian Hu

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Methods Mentioned

BETA
amplicon sequencing
PCR
PCA

Software Mentioned

QIIME
ANOSIM
ggplot2
DEICODE
Quantitative Insights Into Microbial Ecology 2 ( QIIME2
QIIME2
R
ANCOM
Studio
qiime2R

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