Temporally different poly(adenosine diphosphate-ribosylation) signals are required for DNA replication and cell division in early embryos of sea urchins

Journal of Cellular Biochemistry
M ImschenetzkyM Puchi

Abstract

To analyze the temporal relationship of poly(adenosine diphosphate [ADP]-ribosylation) signal with DNA replication and cell divisions, the effect of 3 aminobenzamide (3ABA), an inhibitor of the poly(ADP-ribose)synthetase, was determined in vivo during the first cleavage division of sea urchins. The incorporation of 3H-thymidine into DNA was monitored and cleavage division was examined by light microscopy. The poly(ADP-ribose) neosynthesized on CS histone variants was measured by labeling with 3H-adenosine during the two initial embryonic cell cycles and the inhibitory effect of 3ABA on this poly(ADP-ribosylation) was determined. The results obtained indicate that the CS histone variants are poly(ADP-ribosylated) de novo during the initial cell cycles of embryonic development. The synthesis of poly(ADP-ribose) is decreased but not abolished by 20 mM of 3ABA. The incubation of zygotes in 3ABA at the entrance into S1 phase decreased 3H-thymidine incorporation into DNA in phase S2, while S1 was unaltered. Alternatively, when the same treatment was applied to zygotes at the exit of S1 phase, a block of the first cleavage division and a retardation of S2 phase were observed. The inhibitory effect of 3ABA on both DNA replication and c...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H OkayamaO Hayaishi
Dec 1, 1991·Journal of Cellular Biochemistry·R H GetzenbergD S Coffey
Feb 1, 1991·Archives of Biochemistry and Biophysics·S Tanuma, F Otsuka
Mar 1, 1990·Experimental Cell Research·T BoulikasG Dupuis
Sep 28, 1990·Biochemical and Biophysical Research Communications·C F CesaroneM Orunesu
Nov 5, 1989·Journal of Molecular Biology·J Ménissier-de MurciaG de Murcia
Apr 1, 1989·Biochemical Society Transactions·S Shall
Jun 1, 1989·Experimental Cell Research·M ImschenetzkyR Massone
Jan 15, 1985·European Journal of Biochemistry·A HuletskyG G Poirier
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·U Lönn, S Lönn
Feb 17, 1986·European Journal of Biochemistry·Y TanakaT Kamiya
Aug 1, 1988·The Biochemical Journal·G GoldererP Gröbner
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·F J CasselsG R Vasta
Mar 15, 1985·Biochemical and Biophysical Research Communications·T TaniguchiY Shizuta
Dec 1, 1985·Archives of Biochemistry and Biophysics·K W Adolph
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley
Mar 18, 1982·Nature·D Creissen, S Shall

❮ Previous
Next ❯

Citations

Jun 2, 2007·Journal of Cellular Biochemistry·Claudio IribarrenMaría Imschenetzky
Oct 1, 1995·Journal of Cellular Biochemistry·M ImschenetzkyM Montecino
Apr 1, 1996·Journal of Cellular Biochemistry·M ImschenetzkyM Puchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.