Temporo-spatial requirement of Smad4 in dentin formation

Biochemical and Biophysical Research Communications
Tak-Heun KimEui-Sic Cho

Abstract

The TGF-β/BMP family plays an important role in multiple stages of tooth development. TGF-β/BMP signaling is required for odontoblast differentiation and dentin formation; however, the precise molecular mechanisms underlying dentin formation remain unclear. To address the role of TGF-β/BMP signaling in dentin formation, we analyzed mice in which Smad4, a key intracellular mediator of TGF-β/BMP signaling, was subjected to tissue-specific ablation under the control of Dspp, OC, or Col1a1 promoters. Three independent Smad4 conditional knockout mice exhibited various dentin defects in the crowns and roots of their molars depending on the transactivator. In all mutant molars, crown dentin thickness was thinner than that of the control. In addition, impaired dentin was found in the cervical region and root furcation area. Although the initial differentiation of odontoblasts was normal, odontoblast polarity abruptly decreased and the expression of Col1a1, OC, and Dspp was reduced in the odontoblasts of mutant molars. In Dspp-Cre-mediated Smad4 disruption mice, primary dentin formation was slightly delayed, while secondary dentin formation was severely affected in the cervical region of the molars. These results indicate that TGF-β/BMP...Continue Reading

References

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Jun 7, 2007·Journal of Cell Science·Xiaohong TanXiao Yang
Dec 31, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Wook-Young BaekJung-Eun Kim
Aug 23, 2011·Biochemical and Biophysical Research Communications·Tak-Heun KimEui-Sic Cho
Oct 11, 2011·Journal of Dental Research·W YangJ Gluhak-Heinrich
Jun 29, 2013·International Journal of Oral Science·Audrey RakianStephen E Harris

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