Ten Basic Rules of Antibody Validation

Analytical Chemistry Insights
Michael G Weller

Abstract

The quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able to assess the validation level of an immunochemical reagent. A simple, 1-page checklist is supplied for the practical application of these criteria.

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Citations

May 17, 2019·Advanced Materials·Can DincerFirat Güder
Sep 29, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Matthew Waas, Rebekah L Gundry
Dec 11, 2019·The Journal of Biological Chemistry·Lakshmi Pillai-KastooriAmy R Schutz-Geschwender
Apr 1, 2020·Antibodies·Georg TscheuschnerMichael G Weller
Mar 11, 2020·Frontiers in Chemistry·Ila van KruijsbergenFred van Leeuwen
Nov 28, 2020·Journal of Histotechnology·Katherine CrosbyRoberto D Polakiewicz
Nov 4, 2021·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth

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Methods Mentioned

BETA
immunoprecipitation
surface plasmon resonance
enzyme-linked immunosorbent assay
glycosylations
affinity purification

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