Abstract
To map the preferred cleavage sites of xyloglucan endotransglycosylases (XETs; EC 2.4.1.207) along the donor substrate chain, we incubated the enzymes with tamarind (Tamarindus indica) xyloglucan (donor substrate; approximately 205 kDa; 21 microM) plus the nonasaccharide [(3)H]XLLGol (Gal(2).Xyl(3).Glc(3). [(3)H]glucitol; acceptor substrate; 0.6 microM). After short incubation times, to minimize multiple cleavages, the size of the (3)H-labelled transglycosylation products (determined by gel-permeation chromatography) indicated the positions of the cleavage sites relative to the non-reducing terminus of the donor. There was very little difference between the size profiles of the products formed by any of ten XETs tested [one native XET purified from cauliflower (Brassica oleracea) florets, four native XET isoenzymes purified from etiolated mung-bean (Phaseolus aureus) shoots, native XETs purified from lentil (Lens culinaris) and nasturtium (Tropaeolum majus) seeds, and three insect-cell-produced thale-cress (Arabidopsis thaliana) XETs (EXGT, TCH4 and MERI-5)]. All such product profiles showed a good fit to a model in which the enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selec...Continue Reading
Citations
Feb 16, 2010·Plant Physiology and Biochemistry : PPB·Eva StratilováVladimír Farkas
Apr 25, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Simon PoppingaThomas Speck
Oct 7, 2006·Plant Physiology and Biochemistry : PPB·Z Minic, L Jouanin
Dec 3, 2014·Phytochemistry·Yuan Zhi ShiStephen C Fry
Apr 8, 2015·PloS One·Ye HanJingping Rao
Jun 1, 2016·Frontiers in Plant Science·Ye HanJingping Rao
May 22, 2001·The Plant Journal : for Cell and Molecular Biology·J E Thompson, S C Fry
May 29, 2007·Journal of Experimental Botany·Zoran MinicLise Jouanin
Nov 8, 2007·Planta·Zoë A Popper, Stephen C Fry
Jul 30, 2003·Journal of Experimental Botany·Cecilia N K Suda, Jarbas F Giorgini
Mar 23, 2018·International Journal of Molecular Sciences·Mateusz Majda, Stéphanie Robert
May 22, 2009·The ISME Journal·Renaud BerlemontPablo Power
Jun 16, 2006·The Plant Journal : for Cell and Molecular Biology·Montserrat SaladiéCarmen Catalá
Jul 11, 2006·Plant Molecular Biology·Jaime BecnelJanet Braam
Nov 30, 2007·Planta·Zoran Minic
Jul 20, 2010·Interdisciplinary Sciences, Computational Life Sciences·Qin XuHong Guo
Nov 2, 2005·Nature Reviews. Molecular Cell Biology·Daniel J Cosgrove
May 31, 2019·Annals of Botany·Tu C Nguyen-Phan, Stephen C Fry
Jan 7, 2003·Plant & Cell Physiology·Jocelyn K C RoseKazuhiko Nishitani
Mar 15, 2019·Plant Molecular Biology·Barbora StratilováMaria Hrmova
Jan 22, 2017·The Biochemical Journal·Thomas J Simmons, Stephen C Fry
Jun 15, 2007·The Plant Cell·Martin J BaumannHarry Brumer
Mar 1, 2004·The New Phytologist·Stephen C Fry
May 6, 2004·Journal of the American Chemical Society·Harry BrumerTuula T Teeri