Ten kilobases of 5'-flanking region confers proper regulation of the mouse alcohol dehydrogenase-1 (Adh-1) gene in kidney and adrenal of transgenic mice

Gene
D XieM R Felder

Abstract

The expression profile of the mouse Adh-1 gene, which encodes class I alcohol dehydrogenase enzyme (ADH), is complex and includes tissue specificity and differential hormone responsiveness. Whereas kidney Adh-1 transcription rate is stimulated six- to sevenfold by testosterone treatment, adrenal gland ADH-1 mRNA is reduced to less than 5% of control level within 18 h following hormone administration. Androgen receptor is required for both responses since neither occurs in Tfm mutant mice lacking receptor. Hormonal and tissue-specific aspects of Adh-1 regulation were studied in transgenic mice harboring either of two constructs containing either -2.5 kb or -10 kb of 5'-flanking sequence attached to an Adh-1 minigene. The minigene transcript was expressed in kidney and adrenal tissues, but not liver, in five independent lines harboring a transgene with -2.5 kb of 5'-flanking sequence. Androgen treatment repressed the level of the minigene transcript in adrenal gland, but did not cause induction in kidney. In four lines of transgenic mice carrying the construct with -10 kb of 5'-flanking sequence, the minigene transcript was both repressed in adrenal and induced in kidney by testosterone. These lines have no detectable transgene e...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L Tussey, M R Felder
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H J EdenbergT K Li
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

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Citations

Sep 8, 2004·Endocrine Journal·Masato TakahashiToshihiko Kohno
Sep 11, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R J Haselbeck, G Duester
May 6, 2006·The Journal of Biological Chemistry·Jih-Shyun SuShih-Feng Tsai

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